ArticleScientific reports2018
Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Contribution of tissue clearing and 3D image analysis to in vitro modeling of human cortical development.Scientific reports · 2026Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Neuroendocrine and Developmental Impacts of Early Life Exposure to EDCs.Journal of the Endocrine Society · 2024Article
- Conditional Deletion of KOR (Oprk1) in Kisspeptin Cells Does Not Alter LH Pulses, Puberty, or Fertility in Mice.Endocrinology · 2022Article
- Highlights of neuroanatomical discoveries of the mammalian gonadotropin-releasing hormone system.Journal of neuroendocrinology · 2022Review
- Kiss1 is differentially regulated in male and female mice by the homeodomain transcription factor VAX1.Molecular and cellular endocrinology · 2021Article
- Networking of glucagon-like peptide-1 axons with GnRH neurons in the basal forebrain of male mice revealed by 3DISCO-based immunocytochemistry and optogenetics.Brain structure & function · 2021Article
- Importance of neuroanatomical data from domestic animals to the development and testing of the KNDy hypothesis for GnRH pulse generation.Domestic animal endocrinology · 2020Review
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- Tyrosine-hydroxylase immunoreactivity in the mouse transparent brain and adrenal glands.Journal of neural transmission (Vienna, Austria : 1996) · 2019Review
- KNDy Cells Revisited.Endocrinology · 2018Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Kisspeptin/Neurokinin B/Dynorphin (KNDy) neurons of the arcuate nucleus (ARC) play a key role in the regulation of fertility. The ability to detect features of KNDy neurons that are essential for fertility may require three-dimensional (3D) imaging of the complete population. Recently developed protocols for optical tissue clearing permits 3D imaging of neuronal populations in un-sectioned brains. However, these techniques have largely been described in the mouse brain. We report 3D imaging of the KNDy cell population in the whole rat brain and sheep hypothalamus using immunolabelling and modification of a solvent-based clearing protocol, iDISCO. This study expands the use of optical tissue clearing for multiple mammalian models and provides versatile analysis of KNDy neurons across species. Additionally, we detected a small population of previously unreported kisspeptin neurons in the lateral region of the ovine mediobasal hypothalamus, demonstrating the ability of this technique to detect novel features of the kisspeptin system.
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