ArticleStem cell research & therapy2018
Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Phenotyping of Human Pulp Cells After Cryopreservation.Journal of cellular and molecular medicine · 2026Article
- Evaluation of silver nitroprusside nanoparticles: selective toxicity against MCF-7 cells and in vivo antitumor activity.Discover nano · 2025Article
- Engineered Human Dental Pulp Stem Cells with Promising Potential for Regenerative Medicine.Biotech (Basel (Switzerland)) · 2025Review
- Novel standardized method for inducing medication-related osteonecrosis of the jaw in rats and precise quantitative assessment of pathological outcomes.Odontology · 2025Article
- Microfluidic Devices for Manufacture of Therapeutic Extracellular Vesicles: Advances and Opportunities.Journal of extracellular vesicles · 2025Review
- Enhancing clinical safety in bioengineered-root regeneration: The use of animal component-free medium.Heliyon · 2024Article
- Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation.Bioengineering (Basel, Switzerland) · 2024Article
- Research progress on optimization ofFrontiers in bioengineering and biotechnology · 2024Review
- Functional engineering of human iPSC-derived parasympathetic neurons enhances responsiveness to gastrointestinal hormones.FEBS open bio · 2024Article
- Application of Mesenchymal Stem Cells in Targeted Delivery to the Brain: Potential and Challenges of the Extracellular Vesicle-Based Approach for Brain Tumor Treatment.International journal of molecular sciences · 2021Review
- Comparing Migratory and Mechanical Properties of Human Bone Marrow-Derived Mesenchymal Stem Cells with Colon Cancer Cells In Vitro.Journal of gastrointestinal cancer · 2021Article
- Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review.Restorative dentistry & endodontics · 2021Article
- Hydrogen-Generating Silica Material Prevents UVA-ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents.Antioxidants (Basel, Switzerland) · 2021Article
- Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).PloS one · 2021Article
- Development of DNA polymer films as a drug delivery system for the treatment of oral cancer.Drug delivery and translational research · 2020Article
- Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions.Stem cells translational medicine · 2020Article
- Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture.Stem cell research & therapy · 2020Article
- Rapid differentiation of human dental pulp stem cells to neuron-like cells by high KBiophysics and physicobiology · 2020Article
- Mesenchymal Stem Cell-Derived Extracellular Vesicles: Opportunities and Challenges for Clinical Translation.Frontiers in bioengineering and biotechnology · 2020Review
- The Secretome Derived From Mesenchymal Stromal Cells Cultured in a Xeno-Free Medium Promotes Human Cartilage RecoveryFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrently, ex-vivo handling of stem cells, including transport after harvest and therapeutic preparation, is generally done in culture media containing fetal bovine serum (FBS), which promotes cell attachment, proliferation, and differentiation. However, because of safety concerns associated with the use of FBS, including potential transmission of zoonotic agents and transplant rejection because of the incorporation of foreign proteins into the stem cells, there is a need for xenogeneic serum-free culture media for clinical handling of stem cells.
methodsDental pulp stem cells were derived from wisdom teeth donated by eight healthy volunteers and cultured in xenogeneic serum-free culture medium (XFM) or xenogeneic serum-containing culture medium (SCM). Cells were subjected to morphological, proliferation, karyotype, differentiation, marker expression, cryopreservation, and cytotoxic susceptibility analyses in vitro, as well as transplantation in vivo.
resultsIn primary culture, XFM cells showed lower adhesion and slightly different morphology, although the single-cell size was similar to that of SCM cells. XFM cells exhibited typical mesenchymal stem cell (MSC) characteristics in vitro and in vivo, including marker gene/protein expression, trilineage differentiation potential, and hard, osteo-dentin tissue formation. Additionally, XFM cells maintained a normal karyotype in vitro and nontumorigenic potential in vivo; however, XFM cells were more susceptible to H
conclusionsThis study is the first reporting successful isolation and expansion of an MSC population from donor-derived tissue (dental pulp) under xenogeneic serum-free culture conditions, as well as the application of cryopreservation, using a research strategy based on clinically oriented in-vitro and in-vivo experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.