ArticlePlants (Basel, Switzerland)2018
Imaging and Spectroscopy of Natural Fluorophores in Pine Needles.
Article in Plants (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 87 citations in OpenAlex.
- Video-rate label-free molecular mapping in living plant tissue with a deployable optical encoder.PNAS nexus · 2026Article
- Recent physiological, genetic, and management perspectives on lodging resistance in rice.Planta · 2026Review
- The Research of New Natural Spontaneous FertileInternational journal of molecular sciences · 2026Article
- Unraveling the Effects of Freezing and Frozen Storage Temperatures on Hop Secondary Metabolites and Antioxidants.Antioxidants (Basel, Switzerland) · 2026Article
- Filling in the gaps: evidence of leaf endodermis and vein sheath in gymnosperms.Annals of botany · 2025Article
- An immunohistochemical approach to cell wall polysaccharide specialization in maritime pine (Pinus pinaster) needles.Protoplasma · 2025Article
- Autofluorescence and Metabotyping of Soybean Varieties Using Confocal Laser Microscopy and High-Resolution Mass Spectrometric Approaches.Plants (Basel, Switzerland) · 2025Article
- A novel approach integrating multispectral imaging and machine learning to identify seed maturity and vigor in smooth bromegrass.Plant methods · 2025Article
- Supercritical COPlants (Basel, Switzerland) · 2025Article
- Hygroscopic movements of cone scale of white firFrontiers in plant science · 2025Article
- Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.MethodsX · 2024Article
- Sustainable next-generation color converters fromRSC advances · 2024Article
- Autofluorescence-spectral imaging for rapid and invasive characterization of soybean for pre-germination anaerobic stress tolerance.Frontiers in plant science · 2024Article
- Analysis ofPLOS water · 2023Article
- The Tomato Feruloyl Transferase FHT Promoter Is an Accurate Identifier of Early Development and Stress-Induced Suberization.Plants (Basel, Switzerland) · 2023Article
- Article
- Integrating optical imaging techniques for a novel approach to evaluate Siberian wild rye seed maturity.Frontiers in plant science · 2023Article
- A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM).Toxics · 2022Article
- Review
- An Approach Using Emerging Optical Technologies and Artificial Intelligence Brings New Markers to Evaluate Peanut Seed Quality.Frontiers in plant science · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many plant tissues fluoresce due to the natural fluorophores present in cell walls or within the cell protoplast or lumen. While lignin and chlorophyll are well-known fluorophores, other components are less well characterized. Confocal fluorescence microscopy of fresh or fixed vibratome-cut sections of radiata pine needles revealed the presence of suberin, lignin, ferulate, and flavonoids associated with cell walls as well as several different extractive components and chlorophyll within tissues. Comparison of needles in different physiological states demonstrated the loss of chlorophyll in both chlorotic and necrotic needles. Necrotic needles showed a dramatic change in the fluorescence of extractives within mesophyll cells from ultraviolet (UV) excited weak blue fluorescence to blue excited strong green fluorescence associated with tissue browning. Comparisons were made among fluorophores in terms of optimal excitation, relative brightness compared to lignin, and the effect of pH of mounting medium. Fluorophores in cell walls and extractives in lumens were associated with blue or green emission, compared to the red emission of chlorophyll. Autofluorescence is, therefore, a useful method for comparing the histology of healthy and diseased needles without the need for multiple staining techniques, potentially aiding visual screening of host resistance and disease progression in needle tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.