Evidence map›Paper›PMID 29392386›Full record

ArticleApplied microbiology and biotechnology2018

Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome.

Natalya V Gorshkova, Juliya S Lobanova, Irina L Tokmakova, Sergey V Smirnov, Valerii Z Akhverdyan, Alexander A Krylov, Sergey V Mashko

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Natalya V GorshkovaAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Juliya S LobanovaAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Irina L TokmakovaAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Sergey V SmirnovAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Valerii Z AkhverdyanAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Alexander A KrylovAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545.
Sergey V MashkoAjinomoto-Genetika Research Institute, 1-st Dorozhny proezd, 1-1, Moscow, Russian Federation, 117545. sergey_mashko@agri.ru.
Ajinomoto (Russia) · RUAjinomoto (United States) · USGenetika · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A dual-component Mu-transposition system was modified for the integration/amplification of genes in Corynebacterium. The system consists of two types of plasmids: (i) a non-replicative integrative plasmid that contains the transposing mini-Mu(LR) unit bracketed by the L/R Mu ends or the mini-Mu(LER) unit, which additionally contains the enhancer element, E, and (ii) an integration helper plasmid that expresses the transposition factor genes for MuA and MuB. Efficient transposition in the C. glutamicum chromosome (≈ 2 × 10

Indexed as

Bacteriophage muChromosomes, BacterialDNA Transposable ElementsCorynebacterium glutamicumGene DosageGene EditingGenetics, MicrobialGenetic VectorsPlasmidsRecombination, GeneticDNA Transposable ElementsCre-mediated excisionExcisable enhancerFluorescence proteinsIntrachromosomal amplificationRandom integrationReplicative transposition

Identifiers

PMID29392386
PMCPMC5847225
OpenAlexW2789056536

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.