ArticleScientific reports2018
Saline Accelerates Oxime Reaction with Aldehyde and Keto Substrates at Physiological pH.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Synthesis of Click-Ready Aminooxy-Terminated Poly(ε-caprolactone) Oligomers for Oxime Ligation.Bioconjugate chemistry · 2026Article
- Green Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction Coupled to GC-MS for Simultaneous Determination of Lipid-Peroxidation- and Maillard-Derived Carbonyl Compounds in Plant-Based Beverages.ACS measurement science au · 2025Article
- Engineering protein prenylation: an emerging tool for selective protein modification.Biochemical Society transactions · 2025Review
- Review
- Structure-Reactivity Relationships in a Small Library of Imine-Type Dynamic Covalent Materials: Determination of Rate and Equilibrium Constants Enables Model Prediction and Validation of a Unique Mechanical Softening in Dynamic Hydrogels.Journal of the American Chemical Society · 2024Article
- Bioinspired Synthesis of Allysine for Late-Stage Functionalization of Peptides.Angewandte Chemie (International ed. in English) · 2024Article
- Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity.Chemical reviews · 2023Review
- Direct Identification of Proteolytic Cleavages on Living Cells Using a Glycan-Tethered Peptide Ligase.ACS central science · 2022Article
- Generation and Characterization of Site-Specifically Mono-Ubiquitylated p53.Chembiochem : a European journal of chemical biology · 2022Article
- Eco-friendly methoximation of aromatic aldehydes and ketones using MnClRoyal Society open science · 2021Article
- Pyrazolone ligation-mediated versatile sequential bioconjugations.Chemical science · 2020Article
- Bioconjugation with Aminoalkylhydrazine for Efficient Mass Spectrometry-Based Detection of Small Carbonyl Compounds.ACS omega · 2019Article
- Article
- Exceptionally rapid oxime and hydrazone formation promoted by catalytic amine buffers with low toxicity.Chemical science · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration-dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most biofriendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.
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Registered trials
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