ArticleScientific reports2018
Gene silencing in Tribolium castaneum as a tool for the targeted identification of candidate RNAi targets in crop pests.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 134 citations in OpenAlex.
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- RNA degradomics and proteomics reveal the mechanism of dsProsβ1-mediated proteasome targeting in Psylliodes chrysocephala.Pest management science · 2026Article
- Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus.Pest management science · 2026Article
- Efficacy of Select Gene Targets for the Management of Western Flower Thrips Through RNA Interference.Archives of insect biochemistry and physiology · 2026Article
- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- Microfluidic process-property correlations of dsRNA lipid nanoparticle formulations.Scientific reports · 2026Article
- Distinct Roles of Two UDP-N-Acetylglucosamine Pyrophosphorylase Genes in Chitin Biosynthesis and Molting ofInsects · 2026Article
- Comparative transcriptome analysis of developmental stages and characterization of core RNAi-related genes in the spittlebug Mahanarva fimbriolata.BMC genomics · 2026Article
- Evaluation of dsRNA produced inFrontiers in insect science · 2026Article
- Enhancing the delivery and stability of lipid nanoparticle-dsRNA formulations in the RNAi-recalcitrant fall armyworm (Frontiers in insect science · 2026Article
- RNA interference mediated mortality in Aedes albopictus: a challenging journey toward species-specific vector control.Parasites & vectors · 2025Article
- Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Effective target genes for RNA interference-based management of the cabbage stem flea beetle.Insect molecular biology · 2025Article
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Genome-wide selection of potential target candidates for RNAi against Nilaparvata lugens.BMC genomics · 2024Article
- RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.Scientific reports · 2024Article
- Gene silencing in the aedine cell lines C6/36 and U4.4 using long double-stranded RNA.Parasites & vectors · 2024Article
- Enzyme kinetics of deoxyuridine triphosphatase from Western corn rootworm.BMC research notes · 2023Article
- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNAi shows potential as an agricultural technology for insect control, yet, a relatively low number of robust lethal RNAi targets have been demonstrated to control insects of agricultural interest. In the current study, a selection of lethal RNAi target genes from the iBeetle (Tribolium castaneum) screen were used to demonstrate efficacy of orthologous targets in the economically important coleopteran pests Diabrotica virgifera virgifera and Meligethes aeneus. Transcript orthologs of 50 selected genes were analyzed in D. v. virgifera diet-based RNAi bioassays; 21 of these RNAi targets showed mortality and 36 showed growth inhibition. Low dose injection- and diet-based dsRNA assays in T. castaneum and D. v. virgifera, respectively, enabled the identification of the four highly potent RNAi target genes: Rop, dre4, ncm, and RpII140. Maize was genetically engineered to express dsRNA directed against these prioritized candidate target genes. T
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