ArticleScientific reports2018
Expression and specificity of a chitin deacetylase from the nematophagous fungus Pochonia chlamydosporia potentially involved in pathogenicity.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan.AMB Express · 2025Article
- Rational Design of a Potential New Nematicide Targeting Chitin Deacetylase.Journal of agricultural and food chemistry · 2024Article
- Article
- Article
- In silico and in vitro analysis of an Aspergillus niger chitin deacetylase to decipher its subsite sugar preferences.The Journal of biological chemistry · 2021Article
- Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases.International journal of molecular sciences · 2020Review
- Chitin deacetylases Cod4 and Cod7 are involved in polar growth of Aspergillus fumigatus.MicrobiologyOpen · 2020Article
- Conversion of Chitin to Defined Chitosan Oligomers: Current Status and Future Prospects.Marine drugs · 2019Review
- Molecular Mechanisms of Chitosan Interactions with Fungi and Plants.International journal of molecular sciences · 2019Review
- Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides.Frontiers in bioengineering and biotechnology · 2019Review
- Review
- Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases.International journal of molecular sciences · 2018Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin deacetylases (CDAs) act on chitin polymers and low molecular weight oligomers producing chitosans and chitosan oligosaccharides. Structurally-defined, partially deacetylated chitooligosaccharides produced by enzymatic methods are of current interest as bioactive molecules for a variety of applications. Among Pochonia chlamydosporia (Pc) annotated CDAs, gene pc_2566 was predicted to encode for an extracellular CE4 deacetylase with two CBM18 chitin binding modules. Chitosan formation during nematode egg infection by this nematophagous fungus suggests a role for their CDAs in pathogenicity. The P. chlamydosporia CDA catalytic domain (PcCDA) was expressed in E. coli BL21, recovered from inclusion bodies, and purified by affinity chromatography. It displays deacetylase activity on chitooligosaccharides with a degree of polymerization (DP) larger than 3, generating mono- and di-deacetylated products with a pattern different from those of closely related fungal CDAs. This is the first report of a CDA from a nematophagous fungus. On a DP5 substrate, PcCDA gave a single mono-deacetylated product in the penultimate position from the non-reducing end (ADAAA) which was then transformed into a di-deacetylated product (ADDAA). This novel deacetylation pattern expands our toolbox of specific CDAs for biotechnological applications, and will provide further insights into the determinants of substrate specificity in this family of enzymes.
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