Evidence map›Paper›PMID 29390875›Full record

ArticleCell transplantation2017

Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells.

Patrick Walsh, Vincent Truong, Caitlin Hill, Nicolas D Stoflet, Jessica Baden, Walter C Low, Susan A Keirstead, James R Dutton, Ann M Parr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell transplantation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Patrick Walsh1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Vincent Truong1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Caitlin Hill2 Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
Nicolas D Stoflet1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Jessica Baden1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Walter C Low1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Susan A Keirstead2 Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
James R Dutton2 Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA.
Ann M Parr1 Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.

Funding

University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UL1TR000114 · NCATS · UNIVERSITY OF MINNESOTA · PI BLAZAR, BRUCE R · 2012 to 2015
$35.0M
University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UL1TR002494 · NCATS · UNIVERSITY OF MINNESOTA · PI BLAZAR, BRUCE R, WEISDORF, DANIEL J · 2018 to 2022
$34.9M
Institutional Career Development CoreKL2TR002492 · NCATS · UNIVERSITY OF MINNESOTA · PI INGBAR, DAVID H · 2018 to 2022
$6.3M
NCATS NIH HHS KL2 TR002492NCATS NIH HHS UL1 TR000114NCATS NIH HHS UL1 TR002494
6 · The paper itself

Abstract

The use of defined conditions for derivation, maintenance, and differentiation of human-induced pluripotent stem cells (hiPSCs) provides a superior experimental platform to discover culture responses to differentiation cues and elucidate the basic requirements for cell differentiation and fate restriction. Adoption of defined systems for reprogramming, undifferentiated growth, and differentiation of hiPSCs was found to significantly influence early stage differentiation signaling requirements and temporal kinetics for the production of primitive neuroectoderm. The bone morphogenic protein receptor agonist LDN-193189 was found to be necessary and sufficient for neural induction in a monolayer system with landmark antigens paired box 6 and sex-determining region Y-box 1 appearing within 72 h. Preliminary evidence suggests this neuroepithelium was further differentiated to generate ventral spinal neural progenitors that produced electrophysiologically active neurons in vitro, maintaining viability posttransplantation in an immunocompromised host. Our findings support current developments in the field, demonstrating that adoption of defined reagents for the culture and manipulation of pluripotent stem cells is advantages in terms of simplification and acceleration of differentiation protocols, which will be critical for future clinical translation.

Indexed as

AnimalsCell Culture TechniquesCell DifferentiationCryopreservationElectrophysiologyFemaleHumansImmunohistochemistryInduced Pluripotent Stem CellsKaryotypingKineticsMiceMice, NudeNeural Stem CellsNeuronsPluripotent Stem Cellshuman-induced pluripotent stem cellsneural differentiationventral spinal neurons

Identifiers

PMID29390875
PMCPMC5802631

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.