ArticlePloS one2018
Characterization of chlorophyll binding to LIL3.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Dynamic identification of candidate genes associated with higher tocopherol biosynthesis inFrontiers in plant science · 2025Article
- Genome-wide association identifies a missing hydrolase for tocopherol synthesis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Plant LHC-like proteins show robust folding and static non-photochemical quenching.Nature communications · 2021Article
- Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice.Rice (New York, N.Y.) · 2021Article
- ONE-HELIX PROTEIN1 and 2 Form Heterodimers to Bind Chlorophyll in Photosystem II Biogenesis.Plant physiology · 2020Article
- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids.Nature communications · 2019Article
- A strategy to characterize chlorophyll protein interaction in LIL3.Plant methods · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The light harvesting like protein 3 (LIL 3) from higher plants, has been linked to functions in chlorophyll and tocopherol biosynthesis, photo-protection and chlorophyll transfer. However, the binding of chlorophyll to LIL3 is unclear. We present a reconstitution protocol for chlorophyll binding to LIL3 in DDM micelles. It is shown in the absence of lipids and carotenoids that reconstitution of chlorophyll binding to in vitro expressed LIL3 requires pre-incubation of reaction partners at room temperature. We show chlorophyll a but not chlorophyll b binding to LIL3 at a molar ratio of 1:1. Neither dynamic light scattering nor native PAGE, enabled a discrimination between binding of chlorophyll a and/or b to LIL3.
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