ArticleScientific reports2018
Methylation-based enrichment facilitates low-cost, noninvasive genomic scale sequencing of populations from feces.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 97 citations in OpenAlex.
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- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.Molecular ecology resources · 2026Article
- Genetic legacy and recent cross of two ancient lineages underlie the rebound of the world's rarest primate.Science advances · 2026Article
- Beyond colonoscopy, faecal DNA mutation screening provides a potential and viable path to early colorectal cancer detection.Scientific reports · 2025Article
- A Reassessment of the Genomic Ancestry of the World's Largest Captive Baboon Colony.American journal of primatology · 2025Article
- Validating a remote saliva collection tool for genomic analyses in free ranging dogs.Scientific reports · 2025Article
- Estimating Polar Bear (Ecology and evolution · 2025Article
- Baboons at a Crossroads: Hybridisation Events and Genomic Links of Central Mozambique's Baboons With Papio Neighbors.American journal of biological anthropology · 2025Article
- Rapid molecular species identification of mammalian scat samples using nanopore adaptive sampling.Journal of mammalogy · 2024Article
- FecalSeq enrichment with RAD Sequencing from non-invasive environmental samples holds promise for genetic monitoring of an imperiled lagomorph.Scientific reports · 2024Article
- The Utility of the Koala Scat: A Scoping Review.Biology · 2024Article
- Improving Bacterial Metagenomic Research through Long-Read Sequencing.Microorganisms · 2024Article
- Epigenetic signatures of social status in wild female spotted hyenas (Crocuta crocuta).Communications biology · 2024Article
- From-Toilet-to-Freezer: A Review on Requirements for an Automatic Protocol to Collect and Store Human Fecal Samples for Research Purposes.Biomedicines · 2023Review
- Review
- Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing.Applied and environmental microbiology · 2023Article
- Article
- Dynamic gill and mucus microbiomes during a gill disease episode in farmed Atlantic salmon.Scientific reports · 2022Article
Corrections and comments
- Expression of concern · 2018-12-14Concerns/Issues about Image · Investigation by Journal/Publisher ·
- Correction · 2018-12-19Error in Image ·
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Obtaining high-quality samples from wild animals is a major obstacle for genomic studies of many taxa, particularly at the population level, as collection methods for such samples are typically invasive. DNA from feces is easy to obtain noninvasively, but is dominated by bacterial and other non-host DNA. The high proportion of non-host DNA drastically reduces the efficiency of high-throughput sequencing for host animal genomics. To address this issue, we developed an inexpensive capture method for enriching host DNA from noninvasive fecal samples. Our method exploits natural differences in CpG-methylation density between vertebrate and bacterial genomes to preferentially bind and isolate host DNA from majority-bacterial samples. We demonstrate that the enrichment is robust, efficient, and compatible with downstream library preparation methods useful for population studies (e.g., RADseq). Compared to other enrichment strategies, our method is quick and inexpensive, adding only a negligible cost to sample preparation. In combination with downstream methods such as RADseq, our approach allows for cost-effective and customizable genomic-scale genotyping that was previously feasible in practice only with invasive samples. Because feces are widely available and convenient to collect, our method empowers researchers to explore genomic-scale population-level questions in organisms for which invasive sampling is challenging or undesirable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.