Evidence map›Paper›PMID 29386579›Full record

ArticleScientific reports2018

Plastid super-barcodes as a tool for species discrimination in feather grasses (Poaceae: Stipa).

Katarzyna Krawczyk, Marcin Nobis, Kamil Myszczyński, Ewelina Klichowska, Jakub Sawicki

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed.

  1. Article
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  6. Complete chloroplast genomes of threeFrontiers in plant science · 2025
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  13. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2024
    Article
  14. Review
  15. Decoding the complete chloroplast genome ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023
    Article
  16. Article
  17. The complete chloroplast genome and phylogenetic analysis ofMitochondrial DNA. Part B, Resources · 2023
    Article
  18. Article
  19. Elucidation of the evolutionary history ofFrontiers in plant science · 2023
    Article
  20. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Katarzyna KrawczykDepartment of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. katarzyna.krawczyk@uwm.edu.pl.
Marcin NobisInstitute of Botany, Faculty of Biology, Jagiellonian University, Kraków, Poland.
Kamil MyszczyńskiDepartment of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Ewelina KlichowskaInstitute of Botany, Faculty of Biology, Jagiellonian University, Kraków, Poland.
Jakub SawickiDepartment of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Present study was designed to verify which or if any of plastome loci is a hotspot region for mutations and hence might be useful for molecular species identification in feather grasses. 21 newly sequenced complete plastid genomes representing 19 taxa from the genus of Stipa were analyzed in search of the most variable and the most discriminative loci within Stipa. The results showed that the problem with selecting a good barcode locus for feather grasses lies in the very low level of genetic diversity within its plastome. None of the single chloroplast loci is polymorphic enough to play a role of a barcode or a phylogenetic marker for Stipa. The biggest number of taxa was successfully identified by the analysis of 600 bp long DNA fragment comprising a part of rbcL gene, the complete rbcL-rpl23 spacer and a part of rpl23 gene. The effectiveness of multi-locus barcode composed of six best-performing loci for Stipa (ndhH, rpl23, ndhF-rpl32, rpl32-ccsA, psbK-psbI and petA-psbJ) didn't reach 70% of analyzed taxa. The analysis of complete plastome sequences as a super-barcode for Stipa although much more effective, still didn't allow for discrimination of all the analyzed taxa of feather grasses.

Indexed as

Base PairingBase SequenceChromosome MappingDNA Barcoding, TaxonomicGenetic LociGenetic VariationGenome, PlastidOpen Reading FramesPhylogenyPlastidsPoaceaePolymorphism, Single NucleotideSpecies Specificity

Identifiers

PMID29386579
PMCPMC5792575

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.