ArticleJournal of experimental botany2018
Interference with Clp protease impairs carotenoid accumulation during tomato fruit ripening.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Metabolic cues are the main drivers for the differentiation of chloroplasts into chromoplasts and other plastid types.Journal of experimental botany · 2026Review
- Proteome and Phosphoproteome of Tomato Fruit Identify REDUCED CHLOROPLAST COVERAGE 1a as A Ripening Regulator.Genomics, proteomics & bioinformatics · 2025Article
- Family alliances feeding the carotenoid pathway in tomato.Journal of experimental botany · 2025Review
- Tomato ripening regulator SlSAD8 disturbs nuclear gene transcription and chloroplast-associated protein degradation.Nature plants · 2025Article
- ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more.Molecular horticulture · 2025Review
- The variegatedComputational and structural biotechnology journal · 2024Article
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- Research progress on differentiation and regulation of plant chromoplasts.Molecular biology reports · 2024Review
- Nudix hydrolase 23 post-translationally regulates carotenoid biosynthesis in plants.The Plant cell · 2024Article
- Silencing an ATP-Dependent Caseinolytic Protease Proteolytic Subunit Gene Enhances the Resistance of Rice toInternational journal of molecular sciences · 2024Article
- MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply.Plant communications · 2023Article
- The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas.Frontiers in plant science · 2023Article
- A proteostasis network safeguards the chloroplast proteome.Essays in biochemistry · 2022Review
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Fruit ripening: dynamics and integrated analysis of carotenoids and anthocyanins.BMC plant biology · 2022Review
- "Omics" insights into plastid behavior toward improved carotenoid accumulation.Frontiers in plant science · 2022Review
- Phytoene Synthase: The Key Rate-Limiting Enzyme of Carotenoid Biosynthesis in Plants.Frontiers in plant science · 2022Review
- Overexpression ofFrontiers in plant science · 2022Article
- Protein Aggregation in the Pathogenesis of Ischemic Stroke.Cellular and molecular neurobiology · 2021Review
- An engineered extraplastidial pathway for carotenoid biofortification of leaves.Plant biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profound metabolic and structural changes are required for fleshy green fruits to ripen and become colorful and tasty. In tomato (Solanum lycopersicum), fruit ripening involves the differentiation of chromoplasts, specialized plastids that accumulate carotenoid pigments such as β-carotene (pro-vitamin A) and lycopene. Here, we explored the role of the plastidial Clp protease in chromoplast development and carotenoid accumulation. Ripening-specific silencing of one of the subunits of the Clp proteolytic complex resulted in β-carotene-enriched fruits that appeared orange instead of red when ripe. Clp-defective fruit displayed aberrant chromoplasts and up-regulated expression of nuclear genes encoding the tomato homologs of Orange (OR) and ClpB3 chaperones, most probably to deal with misfolded and aggregated proteins that could not be degraded by the Clp protease. ClpB3 and OR chaperones protect the carotenoid biosynthetic enzymes deoxyxylulose 5-phosphate synthase and phytoene synthase, respectively, from degradation, whereas OR chaperones additionally promote chromoplast differentiation by preventing the degradation of carotenoids such as β-carotene. We conclude that the Clp protease contributes to the differentiation of chloroplasts into chromoplasts during tomato fruit ripening, acting in co-ordination with specific chaperones that alleviate protein folding stress, promote enzyme stability and accumulation, and prevent carotenoid degradation.
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