ArticleNucleic acids research2018
Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Evidence for dual pathways of Tc1/mariner domestication in Drosophila.BMC biology · 2026Article
- Discovery of a chimeric transposase-transposon system for advanced genome engineering.iScience · 2026Article
- A developmental condensin I complex assists the Paramecium PiggyMac domesticated transposase during programmed DNA elimination.Nucleic acids research · 2026Article
- ThebioRxiv : the preprint server for biology · 2025Article
- Exploring transposable elements: new horizons in cancer diagnostics and therapeutics.Mobile DNA · 2025Review
- Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends.Nature communications · 2025Article
- Two paralogous PHD finger proteins participate in natural genome editing in Paramecium tetraurelia.Journal of cell science · 2024Article
- Article
- Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells.Nucleic acids research · 2022Article
- A Novel Gene Controls a New Structure: PiggyBac Transposable Element-Derived 1, Unique to Mammals, Controls Mammal-Specific Neuronal Paraspeckles.Molecular biology and evolution · 2022Article
- Functional Characterization of the N-Terminal Disordered Region of theInternational journal of molecular sciences · 2022Article
- Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes.NAR genomics and bioinformatics · 2022Article
- Transposase-CRISPR mediated targeted integration (TransCRISTI) in the human genome.Scientific reports · 2022Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases.Mobile DNA · 2021Article
- The C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition.Journal of molecular biology · 2021Article
- The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2.Journal of molecular biology · 2021Article
- Structural basis of seamless excision and specific targeting by piggyBac transposase.Nature communications · 2020Article
- RNA-guidedSynthetic biology (Oxford, England) · 2019Article
- Article
Corrections and comments
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Authors and funding
16 authors at 8 institutions in 2 countries.
Funding
Abstract
The piggyBac transposase (PB) is distinguished by its activity and utility in genome engineering, especially in humans where it has highly promising therapeutic potential. Little is known, however, about the structure-function relationships of the different domains of PB. Here, we demonstrate in vitro and in vivo that its C-terminal Cysteine-Rich Domain (CRD) is essential for DNA breakage, joining and transposition and that it binds to specific DNA sequences in the left and right transposon ends, and to an additional unexpectedly internal site at the left end. Using NMR, we show that the CRD adopts the specific fold of the cross-brace zinc finger protein family. We determine the interaction interfaces between the CRD and its target, the 5'-TGCGT-3'/3'-ACGCA-5' motifs found in the left, left internal and right transposon ends, and use NMR results to propose docking models for the complex, which are consistent with our site-directed mutagenesis data. Our results provide support for a model of the PB/DNA interactions in the context of the transpososome, which will be useful for the rational design of PB mutants with increased activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.