Evidence map›Paper›PMID 29385522›Full record

ArticleNucleic acids research2018

The hepatitis C viral nonstructural protein 5A stabilizes growth-regulatory human transcripts.

Liang Guo, Suresh D Sharma, Jose D Debes, Daniel Beisang, Bernd Rattenbacher, Irina Vlasova-St Louis, Darin L Wiesner, Craig E Cameron, Paul R Bohjanen

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Liang GuoDepartment of Medicine, Division of Infectious Diseases and International Medicine, Program in Infection and Immunity, University of Minnesota, Minneapolis, MN 55455, USA.
Suresh D SharmaDepartment of Biochemistry & Molecular Biology, The Pennsylvania State University 201 Althouse Laboratory, University Park, PA 16802, USA.
Jose D DebesDepartment of Medicine, Division of Infectious Diseases and International Medicine, Program in Infection and Immunity, University of Minnesota, Minneapolis, MN 55455, USA.
Daniel BeisangDepartment of Medicine, Division of Infectious Diseases and International Medicine, Program in Infection and Immunity, University of Minnesota, Minneapolis, MN 55455, USA.
Bernd RattenbacherDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Irina Vlasova-St LouisDepartment of Medicine, Division of Infectious Diseases and International Medicine, Program in Infection and Immunity, University of Minnesota, Minneapolis, MN 55455, USA.
Darin L WiesnerDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Craig E CameronDepartment of Biochemistry & Molecular Biology, The Pennsylvania State University 201 Althouse Laboratory, University Park, PA 16802, USA.
Paul R BohjanenDepartment of Medicine, Division of Infectious Diseases and International Medicine, Program in Infection and Immunity, University of Minnesota, Minneapolis, MN 55455, USA.
University of Minnesota · USPennsylvania State University · US

Funding

TRAINING-PULMONARY CELL &MOLECULAR BIOLOGY &PHYSIOLOGYT32HL007741 · NHLBI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DUDLEY, R. ADAMS, INGBAR, DAVID H · 1994 to 2023
$13.9M
IMMUNOLOGY TRAINING PROGRAMT32AI007313 · NIAID · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Carla Rothlin · 1989 to 2026
$5.7M
Minnesota Training Program in VirologyT32AI083196 · NIAID · UNIVERSITY OF MINNESOTA · PI Louis M Mansky · 2010 to 2026
$3.2M
Novel Regulators of T Cell mRNA DecayR01AI072068 · NIAID · UNIVERSITY OF MINNESOTA · PI BOHJANEN, PAUL R · 2008 to 2011
$1.3M
Outcomes of Cryptococcal Meningitis in UgandaK24AI096925 · NIAID · UNIVERSITY OF MINNESOTA · PI BOHJANEN, PAUL R · 2011 to 2015
$1.0M
Regulation of T Lymphocyte mRNA DegradationK02AI052170 · NIAID · UNIVERSITY OF MINNESOTA TWIN CITIES · PI BOHJANEN, PAUL R · 2002 to 2006
$539k
mRNA Decay in T Lymphocyte ActivationR56AI057484 · NIAID · UNIVERSITY OF MINNESOTA · PI BOHJANEN, PAUL R · 2006 to 2006
$324k
NHLBI NIH HHS T32 HL007741NIAID NIH HHS K02 AI052170NIAID NIH HHS K24 AI096925NIAID NIH HHS R01 AI072068NIAID NIH HHS R56 AI057484NIAID NIH HHS T32 AI007313NIAID NIH HHS T32 AI083196
6 · The paper itself

Abstract

Numerous mammalian proto-oncogene and other growth-regulatory transcripts are upregulated in malignancy due to abnormal mRNA stabilization. In hepatoma cells expressing a hepatitis C virus (HCV) subgenomic replicon, we found that the viral nonstructural protein 5A (NS5A), a protein known to bind to viral RNA, also bound specifically to human cellular transcripts that encode regulators of cell growth and apoptosis, and this binding correlated with transcript stabilization. An important subset of human NS5A-target transcripts contained GU-rich elements, sequences known to destabilize mRNA. We found that NS5A bound to GU-rich elements in vitro and in cells. Mutation of the NS5A zinc finger abrogated its GU-rich element-binding and mRNA stabilizing activities. Overall, we identified a molecular mechanism whereby HCV manipulates host gene expression by stabilizing host transcripts in a manner that would promote growth and prevent death of virus-infected cells, allowing the virus to establish chronic infection and lead to the development of hepatocellular carcinoma.

Indexed as

RNA StabilityCell Line, TumorHeLa CellsHepacivirusHumansProto-Oncogene MasRNA-Dependent RNA PolymeraseRNA, MessengerViral Nonstructural ProteinsMAS1 protein, humanNS-5 protein, hepatitis C virusProto-Oncogene MasRNA-Dependent RNA PolymeraseRNA, MessengerViral Nonstructural Proteins

Identifiers

PMID29385522
PMCPMC5861452
OpenAlexW2790464280

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.