ArticleGigaScience2018
Giardia secretome highlights secreted tenascins as a key component of pathogenesis.
Article in GigaScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Global epidemiology of Giardia duodenalis infection in cancer patients: a systematic review and meta-analysis.International health · 2022Pooled it
- Pathogenicity and virulence ofVirulence · 2026Review
- Review
- The differential roles of Giardia duodenalis-secreted tenascins in inducing intestinal epithelial cell apoptosis and the attributed EGFR-STAT3 axis regulation.PLoS neglected tropical diseases · 2026Article
- Iron Regulatory Mechanism IRE/IRP-like in Two Protozoa of Importance to Human Health,Pathogens (Basel, Switzerland) · 2026Review
- Genotype-Specific Small EVs Released by Giardia lamblia Act as Mediators of Phenotypic Adaptation Under Metronidazole-Induced Stress.Journal of extracellular vesicles · 2025Article
- Annexin-Membrane Interactions Across Eukaryotic Domains of Life-A Comparative Approach.International journal of molecular sciences · 2025Review
- The pathogenic responses elicited during exposure of human intestinal cell line with Giardia duodenalis excretory-secretory products and the potential attributed endocytosis mechanism.Medical microbiology and immunology · 2024Article
- Expanded gene and taxon sampling of diplomonads shows multiple switches to parasitic and free-living lifestyle.BMC biology · 2024Article
- Insights into Peptidyl-ProlylPathogens (Basel, Switzerland) · 2024Review
- Current Understanding ofCurrent tropical medicine reports · 2024Article
- A core UPS molecular complement implicates unique endocytic compartments at the parasite-host interface inVirulence · 2023Article
- Glycan Complexity and Heterogeneity of Glycoproteins in Somatic Extracts and Secretome of the Infective Stage of the Helminth Fasciola hepatica.Molecular & cellular proteomics : MCP · 2023Article
- Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens-Factors Linked to Virulence and Pathogenicity.International journal of molecular sciences · 2023Review
- The Exosome-like Vesicles ofInternational journal of molecular sciences · 2023Article
- Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection.PLoS pathogens · 2023Article
- ROS-AMPK/mTOR-dependent enterocyte autophagy is involved in the regulation ofFrontiers in immunology · 2023Article
- Specific TLR-mediated HSP70 activation plays a potential role in host defense against the intestinal parasiteFrontiers in microbiology · 2023Article
- Protozoa-Derived Extracellular Vesicles on Intercellular Communication with Special Emphasis onMicroorganisms · 2022Review
- Application of Proteomics to the Study of the Therapeutics and Pathogenicity ofDiagnostics (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Giardia is a protozoan parasite of public health relevance that causes gastroenteritis in a wide range of hosts. Two genetically distinct lineages (assemblages A and B) are responsible for the human disease. Although it is clear that differences in virulence occur, the pathogenesis and virulence of Giardia remain poorly understood. Results: The genome of Giardia is believed to contain open reading frames that could encode as many as 6000 proteins. By successfully applying quantitative proteomic analyses to the whole parasite and to the supernatants derived from parasite culture of assemblages A and B, we confirm expression of ∼1600 proteins from each assemblage, the vast majority of which are common to both lineages. To look for signature enrichment of secreted proteins, we considered the ratio of proteins in the supernatant compared with the pellet, which defined a small group of enriched proteins, putatively secreted at a steady state by cultured growing trophozoites of both assemblages. This secretome is enriched with proteins annotated to have N-terminal signal peptide. The most abundant secreted proteins include known virulence factors such as cathepsin B cysteine proteases and members of a Giardia superfamily of cysteine-rich proteins that comprise variant surface proteins, high-cysteine membrane proteins, and a new class of virulence factors, the Giardia tenascins. We demonstrate that physiological function of human enteric epithelial cells is disrupted by such soluble factors even in the absence of the trophozoites. Conclusions: We are able to propose a straightforward model of Giardia pathogenesis incorporating key roles for the major Giardia-derived soluble mediators.
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Registered trials
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