Evidence map›Paper›PMID 29383682›Full record

ArticleMolecular neurobiology2018

Disruption of De Novo Serine Synthesis in Müller Cells Induced Mitochondrial Dysfunction and Aggravated Oxidative Damage.

Ting Zhang, Mark C Gillies, Michele C Madigan, Weiyong Shen, Jianhai Du, Ulrike Grünert, Fanfan Zhou, Michelle Yam, Ling Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 64 citations in OpenAlex.

  1. Review
  2. Macular telangiectasia type 2: An update.The Journal of international medical research · 2026
    Review
  3. Mechanical Loading Induces Distinct and Shared Responses in Endothelial and Muscle Cells and Reveals Exercise-Like Molecular Profiles.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. L-serine metabolic regulation and host respiratory homeostasis.Frontiers in cellular and infection microbiology · 2025
    Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Ting ZhangState Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Mark C GilliesSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Michele C MadiganSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Weiyong ShenSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Jianhai DuWest Virginia University Health Sciences Center, Morgantown, WV, USA.
Ulrike GrünertSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Fanfan ZhouFaculty of Pharmacy, The University of Sydney, Sydney, NSW, Australia.
Michelle YamSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Ling ZhuSave Sight Institute, The University of Sydney, 8 Macquarie Street, Sydney, NSW, 2000, Australia. ling.zhu@sydney.edu.au.ORCID http://orcid.org/0000-0003-0776-1630
The University of Sydney · AUWest Virginia University · US

Funding

Human RPE metabolism and metabolite transportR01EY026030 · NEI · UNIVERSITY OF WASHINGTON · PI CHAO, JENNIFER RAYMING, DU, JIANHAI · 2016 to 2020
$2.1M
NEI NIH HHS R01 EY026030
6 · The paper itself

Abstract

De novo serine synthesis plays important roles in normal mitochondrial function and cellular anti-oxidative capacity. It is reported to be mainly activated in glial cells of the central nervous system, but its role in retinal Müller glia remains unclear. In this study, we inhibited de novo serine synthesis using CBR-5884, a specific inhibitor of phosphoglycerate dehydrogenase (PHGDH, a rate limiting enzyme in de novo serine metabolism) in MIO-M1 cells (immortalized human Müller cells) and huPMCs (human primary Müller cells) under mild oxidative stress. Alamar blue and LDH (lactate dehydrogenase) assays showed significantly reduced metabolic activities and increased cellular damage of Müller cells, when exposed to CBR-5884 accompanied by mild oxidative stress; however, CBR-5884 alone had little effect. The increased cellular damage was partially reversed by supplementation with exogenous serine/glycine. HSP72 (an oxidative stress marker) and reactive oxygen species (ROS) levels were significantly increased; glutathione and NADPH/NADP

Indexed as

Oxidative StressAdenosine TriphosphateAgedChaperonin 60Ependymoglial CellsGlutathioneHSP72 Heat-Shock ProteinsHumansMiddle AgedMitochondriaNADPPhosphoglycerate DehydrogenaseReactive Oxygen SpeciesSerineUp-RegulationAdenosine TriphosphateChaperonin 60GlutathioneHSP72 Heat-Shock ProteinsNADPPhosphoglycerate DehydrogenaseReactive Oxygen SpeciesSerineDe novo serine synthesisGlutathioneMitochondrial dysfunctionMüller cellsOxidative stressPhosphoglycerate dehydrogenase

Identifiers

PMID29383682
OpenAlexW2789474544

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.