ArticleScientific reports2018
Red fluorescent cAMP indicator with increased affinity and expanded dynamic range.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
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- Collective surfing of single cells on a chemo-attractant wave using multiscale Eulerian velocity vector field.Scientific reports · 2026Article
- Red-Shifted Epac-Based FRET cAMP Sensors for All-Optical cAMP Control and Multiparameter Imaging.Cells · 2026Article
- A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.Nature communications · 2026Article
- Targeting prostaglandin ENature communications · 2025Article
- Probing spatiotemporally organized GPCR signaling using genetically encoded molecular tools.Experimental & molecular medicine · 2025Review
- Fluorescent sensors for intracellular signaling in the brain: imaging neurons, glia, and vascular cells.Neurophotonics · 2025Article
- Optogenetic control of cAMP oscillations reveals frequency-selective transcription factor dynamics in Dictyostelium.Development (Cambridge, England) · 2025Article
- Multi-color fluorescence live-cell imaging in Dictyostelium discoideum.Cell structure and function · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.Communications biology · 2024Article
- Analysis of the singularity cells controlling the pattern formation in multi-cellular systems.Biophysics and physicobiology · 2024Article
- Adenylyl cyclase isoforms 5 and 6 in the cardiovascular system: complex regulation and divergent roles.Frontiers in pharmacology · 2024Review
- Subcellular second messenger networks drive distinct repellent-induced axon behaviors.Nature communications · 2023Article
- Essential role of P-glycoprotein in the mechanism of action of oliceridine.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Essential role of the mitochondrial NaCell reports · 2022Article
- Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.Nature methods · 2022Article
- A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging.Nature communications · 2022Article
- A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.ACS chemical biology · 2022Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cAMP is one of the most important second messengers in biological processes. Cellular dynamics of cAMP have been investigated using a series of fluorescent indicators; however, their sensitivity was sub-optimal for detecting cAMP dynamics at a low concentration range, due to a low ligand affinity and/or poor dynamic range. Seeking an indicator with improved detection sensitivity, we performed insertion screening of circularly permuted mApple, a red fluorescent protein, into the cAMP-binding motif of PKA regulatory subunit Iα and developed an improved cAMP indicator named R-FlincA (Red Fluorescent indicator for cAMP). Its increased affinity (K
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