ArticleScientific reports2018
Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05964920 (Does Human Skeletal Muscle Possess an Epigenetic Memory of Testosterone?), which is not on this map. Cited by 165 papers, 5 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Does Human Skeletal Muscle Possess an Epigenetic Memory of Testosterone?
Who cites it
165 citing papers in PubMed, 5 syntheses or guidelines pooled it, 318 citations in OpenAlex.
- Exercise is associated with younger methylome and transcriptome profiles in human skeletal muscle.Aging cell · 2024Pooled it
- Myonuclear permanence in skeletal muscle memory: a systematic review and meta-analysis of human and animal studies.Journal of cachexia, sarcopenia and muscle · 2022Pooled it
- The Effect of Intensity, Frequency, Duration and Volume of Physical Activity in Children and Adolescents on Skeletal Muscle Fitness: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.International journal of environmental research and public health · 2021Pooled it
- Meta-analysis of genome-wide DNA methylation and integrative omics of age in human skeletal muscle.Journal of cachexia, sarcopenia and muscle · 2021Pooled it
- Epigenetic changes in healthy human skeletal muscle following exercise- a systematic review.Epigenetics · 2019Pooled it
- Repeated Resistance Training Reveals the Reproducibility of Muscle Strength and Size Responses Within Individuals.European journal of sport science · 2025Trial
- Trial
- Acute normobaric hypoxia blunts contraction-mediated mTORC1- and JNK-signaling in human skeletal muscle.Acta physiologica (Oxford, England) · 2022Trial
- Mechanical loading of bioengineered skeletal muscle in vitro recapitulates gene expression signatures of resistance exercise in vivo.Journal of cellular physiology · 2021Trial
- Muscle transcriptional networks linked to resistance exercise training hypertrophic response heterogeneity.Physiological genomics · 2021Trial
- Trial
- Lifelong physical activity is associated with promoter hypomethylation of genes involved in metabolism, myogenesis, contractile properties and oxidative stress resistance in aged human skeletal muscle.Scientific reports · 2019Trial
- Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation.Epigenetics · 2019Trial
- Skeletal muscle ceramides and relationship with insulin sensitivity after 2 weeks of simulated sedentary behaviour and recovery in healthy older adults.The Journal of physiology · 2018Trial
- Aging concomitantly reduces skeletal muscle proteome plasticity and hypertrophic responses to resistance training in male adults.Physiological reports · 2026Article
- Multi-omics identifies promoter methylation and gene expression changes associated with human skeletal muscle atrophy.Experimental physiology · 2026Article
- Basal Tone, Muscle Memory and Denervation: Pathophysiological Foundations of Abdominal Wall Hernia Development and Recovery.Pathophysiology : the official journal of the International Society for Pathophysiology · 2026Review
- Effects of Clenbuterol on Body Composition and Strength Following Resistance Training, Detraining, and Retraining in Healthy Adults: A Randomized Controlled Trial.Sports medicine (Auckland, N.Z.) · 2026Article
- Review
- Influence of Chromium Propionate Supplementation in Ewes During the Gestational Phase on the Regulation of Genes Associated with Growth and Metabolism in the Offspring.Biological trace element research · 2026Article
105 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is unknown if adult human skeletal muscle has an epigenetic memory of earlier encounters with growth. We report, for the first time in humans, genome-wide DNA methylation (850,000 CpGs) and gene expression analysis after muscle hypertrophy (loading), return of muscle mass to baseline (unloading), followed by later hypertrophy (reloading). We discovered increased frequency of hypomethylation across the genome after reloading (18,816 CpGs) versus earlier loading (9,153 CpG sites). We also identified AXIN1, GRIK2, CAMK4, TRAF1 as hypomethylated genes with enhanced expression after loading that maintained their hypomethylated status even during unloading where muscle mass returned to control levels, indicating a memory of these genes methylation signatures following earlier hypertrophy. Further, UBR5, RPL35a, HEG1, PLA2G16, SETD3 displayed hypomethylation and enhanced gene expression following loading, and demonstrated the largest increases in hypomethylation, gene expression and muscle mass after later reloading, indicating an epigenetic memory in these genes. Finally, genes; GRIK2, TRAF1, BICC1, STAG1 were epigenetically sensitive to acute exercise demonstrating hypomethylation after a single bout of resistance exercise that was maintained 22 weeks later with the largest increase in gene expression and muscle mass after reloading. Overall, we identify an important epigenetic role for a number of largely unstudied genes in muscle hypertrophy/memory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.