ArticleScientific reports2018
Convergent and Divergent Migratory Patterns of Human Neutrophils inside Microfluidic Mazes.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- Modelling chemotaxis of branched cells in complex environments provides insights into immune cell navigation.PLoS computational biology · 2026Article
- Signaling dynamics distinguish high- and low-priority neutrophil chemoattractant receptors.Science signaling · 2023Article
- Actuation of single downstream nodes in growth factor network steers immune cell migration.Developmental cell · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Neutrophil dysfunction in cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2021Article
- A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation.Scientific reports · 2021Article
- Human Neutrophils Respond to Complement Activation and Inhibition in Microfluidic Devices.Frontiers in immunology · 2021Article
- Analyzing Inter-Leukocyte Communication and MigrationFrontiers in immunology · 2021Article
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Chips-on-a-plate device for monitoring cellular migration in a microchannel-based intestinal follicle-associated epithelium model.Biomicrofluidics · 2019Article
- Concepts of GPCR-controlled navigation in the immune system.Immunological reviews · 2019Review
- Towards the development of human immune-system-on-a-chip platforms.Drug discovery today · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Neutrophils are key cellular components of the innate immune response and characteristically migrate from the blood towards and throughout tissues. Their migratory process is complex, guided by multiple chemoattractants released from injured tissues and microbes. How neutrophils integrate the various signals in the tissue microenvironment and mount effective responses is not fully understood. Here, we employed microfluidic mazes that replicate features of interstitial spaces and chemoattractant gradients within tissues to analyze the migration patterns of human neutrophils. We find that neutrophils respond to LTB4 and fMLF gradients with highly directional migration patterns and converge towards the source of chemoattractant. We named this directed migration pattern convergent. Moreover, neutrophils respond to gradients of C5a and IL-8 with a low-directionality migration pattern and disperse within mazes. We named this alternative migration pattern divergent. Inhibitors of MAP kinase and PI-3 kinase signaling pathways do not alter either convergent or divergent migration patterns, but reduce the number of responding neutrophils. Overlapping gradients of chemoattractants conserve the convergent and divergent migration patterns corresponding to each chemoattractant and have additive effects on the number of neutrophils migrating. These results suggest that convergent and divergent neutrophil migration-patterns are the result of simultaneous activation of multiple signaling pathways.
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