ArticleJournal of industrial microbiology & biotechnology2018
Rational design of a synthetic Entner-Doudoroff pathway for enhancing glucose transformation to isobutanol in Escherichia coli.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Regulating microbial cell factories to resist ROS stress in chemical production.Archives of microbiology · 2026Review
- Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression.Current issues in molecular biology · 2026Review
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- EngineeringMicroorganisms · 2023Article
- Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.Biotechnology for biofuels and bioproducts · 2023Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Microbial engineering for the production of isobutanol: current status and future directions.Bioengineered · 2021Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Coexistence of the Entner-Doudoroff and Embden-Meyerhof-Parnas pathways enhances glucose consumption of ethanol-producing Corynebacterium glutamicum.Biotechnology for biofuels · 2021Article
- Engineering Improves Enzymatic Synthesis of L-Tryptophan by Tryptophan Synthase fromMicroorganisms · 2020Article
- Synthetic Biology and Metabolic Engineering EmployingFrontiers in bioengineering and biotechnology · 2020Review
- Reconstruction of metabolic pathway for isobutanol production in Escherichia coli.Microbial cell factories · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Isobutanol as a more desirable biofuel has attracted much attention. In our previous work, an isobutanol-producing strain Escherichia coli LA09 had been obtained by rational redox status improvement under guidance of the genome-scale metabolic model. However, the low transformation from sugar to isobutanol is a limiting factor for isobutanol production by E. coli LA09. In this study, the intracellular metabolic profiles of the isobutanol-producing E. coli LA09 with different initial glucose concentrations were investigated and the metabolic reaction of fructose 6-phosphate to 1, 6-diphosphate fructose in glycolytic pathway was identified as the rate-limiting step of glucose transformation. Thus, redesigned carbon catabolism was implemented by altering flux of sugar metabolism. Here, the heterologous Entner-Doudoroff (ED) pathway from Zymomonas mobilis was constructed, and the adaptation of upper and lower parts of ED pathway was further improved with artificial promoters to alleviate the accumulation of toxic intermediate metabolite 2-keto-3-deoxy-6-phospho-gluconate (KDPG). Finally, the best isobutanol-producing E. coli ED02 with higher glucose transformation and isobutanol production was obtained. In the fermentation of strain E. coli ED02 with 45 g/L initial glucose, the isobutanol titer, yield and average producing rate were, respectively, increased by 56.8, 47.4 and 88.1% to 13.67 g/L, 0.50 C-mol/C-mol and 0.456 g/(L × h) in a shorter time of 30 h, compared with that of the starting strain E. coli LA09.
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