ArticleJournal of industrial microbiology & biotechnology2018
Membrane engineering via trans-unsaturated fatty acids production improves succinic acid production in Mannheimia succiniciproducens.
Article in Journal of industrial microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Perspectives for Advancing Biotechnological Succinic Acid Production.Microbial biotechnology · 2026Review
- Genome-Scale Engineering Importing Property of Escherichia coli for Improving Production of 3-Hydroxypropionic Acid.Microbial biotechnology · 2026Article
- Succinic Acid Production by Engineered Mannheimia succiniciproducens and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis.Biotechnology and bioengineering · 2025Article
- Towards Bacterial Resistance via the Membrane Strategy: Enzymatic, Biophysical and Biomimetic Studies of the Lipid cis-trans Isomerase of Pseudomonas aeruginosa.Chembiochem : a European journal of chemical biology · 2025Article
- High-level succinic acid production by overexpressing a magnesium transporter inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry.Biotechnology for biofuels and bioproducts · 2024Review
- Sustainable production and degradation of plastics using microbes.Nature microbiology · 2023Review
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories.Microbial cell factories · 2021Review
- Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase.Nature communications · 2020Article
- Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance.Journal of industrial microbiology & biotechnology · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Engineering of microorganisms to produce desired bio-products with high titer, yield, and productivity is often limited by product toxicity. This is also true for succinic acid (SA), a four carbon dicarboxylic acid of industrial importance. Acid products often cause product toxicity to cells through several different factors, membrane damage being one of the primary factors. In this study, cis-trans isomerase from Pseudomonas aeruginosa was expressed in Mannheimia succiniciproducens to produce trans-unsaturated fatty acid (TUFA) and to reinforce the cell membrane of M. succiniciproducens. The engineered strain showed significant decrease in membrane fluidity as production of TUFA enabled tight packing of fatty acids, which made cells to possess more rigid cell membrane. As a result, the membrane-engineered M. succiniciproducens strain showed higher tolerance toward SA and increased production of SA compared with the control strain without membrane engineering. The membrane engineering approach employed in this study will be useful for increasing tolerance to, and consequently enhancing production of acid products.
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