Evidence map›Paper›PMID 29379500›Full record

ArticleFrontiers in immunology2017

Intravital Microscopic Evaluation of the Effects of a CXCR2 Antagonist in a Model of Liver Ischemia Reperfusion Injury in Mice.

Thiago Henrique Caldeira de Oliveira, Pedro Elias Marques, Fariba Poosti, Pieter Ruytinx, Flávio Almeida Amaral, Laura Brandolini, Marcello Allegretti, Paul Proost, Mauro Martins Teixeira

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Neutrophil diversity and function in health and disease.Signal transduction and targeted therapy · 2024
    Review
  6. Review
  7. Review
  8. Ovarian hormones influence immune response to liver ischemia-reperfusion.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023
    Article
  9. Immune response associated with ischemia and reperfusion injury during organ transplantation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. The role of neutrophil extracellular traps and TLR signaling in skeletal muscle ischemia reperfusion injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
  16. [Protective effect of serine methyltransferase against hepatic ischemia-reperfusion injury in mice].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2020
    Article
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Thiago Henrique Caldeira de OliveiraImmunopharmacology Laboratory, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Pedro Elias MarquesProgram in Cell Biology, The Hospital of Sick Children, Toronto, ON, Canada.
Fariba PoostiLaboratory of Molecular Immunology, Department of Microbiology and Immunology, Rega Institute, Catholic University of Leuven, Leuven, Belgium.
Pieter RuytinxLaboratory of Molecular Immunology, Department of Microbiology and Immunology, Rega Institute, Catholic University of Leuven, Leuven, Belgium.
Flávio Almeida AmaralImmunopharmacology Laboratory, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Laura BrandoliniDompé farmaceutici S.p.A., Milano, Italy.
Marcello AllegrettiDompé farmaceutici S.p.A., Milano, Italy.
Paul ProostLaboratory of Molecular Immunology, Department of Microbiology and Immunology, Rega Institute, Catholic University of Leuven, Leuven, Belgium.
Mauro Martins TeixeiraImmunopharmacology Laboratory, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Dompé (Italy) · ITKU Leuven · BERega Institute for Medical Research · BEUniversidade Federal de Minas Gerais · BRHospital for Sick Children · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIschemia-reperfusion (IR) is a major contributor to graft rejection after liver transplantation. During IR injury, an intense inflammatory process occurs in the liver. Neutrophils are considered central players in the events that lead to liver injury. CXC chemokines mediate hepatic inflammation following reperfusion. However, few studies have demonstrated in real-time the behavior of recruited neutrophils. We used confocal intravital microscopy (IVM) to image neutrophil migration in the liver and to analyze in real-time parameters of neutrophil recruitment in the inflamed tissue in animals treated or not with reparixin, an allosteric antagonist of CXCR1/2 receptors. MATERIALS AND

methodsWT and LysM-eGFP mice treated with reparixin or saline were subjected to 60 min of ischemia followed by different times of reperfusion. Mice received Sytox orange intravenously to show necrotic DNA in IVM. The effect of reparixin on parameters of local and systemic reperfusion-induced injury was also investigated.

resultsIR induced liver injury and inflammation, as evidenced by high levels of alanine aminotransferase and myeloperoxidase activity, chemokine and cytokine production, and histological outcome. Treatment with reparixin significantly decreased neutrophil influx. Moreover, reparixin effectively suppressed the increase in serum concentrations of TNF-α, IL-6, and CCL3, and the reperfusion-associated tissue damage. The number of neutrophils in the liver increased between 6 and 24 h of reperfusion, whereas the distance traveled, velocity, neutrophil size and shape, and cluster formation reached a maximum 6 h after reperfusion and then decreased gradually.

conclusionImaging of the liver by confocal IVM was successfully implemented to describe neutrophil behavior

Indexed as

chemokinesCXCR2hepatic ischemia–reperfusionneutrophil-mediated liver injuryneutrophil migration

Identifiers

PMID29379500
PMCPMC5770890
OpenAlexW2781776928

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.