ArticleNature microbiology2018
Spontaneous CRISPR loci generation in vivo by non-canonical spacer integration.
Article in Nature microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Toward DNA-Based Recording of Biological Processes.International journal of molecular sciences · 2024Review
- Article
- Assessing spacer acquisition rates inFrontiers in microbiology · 2024Article
- DNA Motifs and an Accessory CRISPR Factor Determine Cas1 Binding and Integration Activity inInternational journal of molecular sciences · 2022Article
- Creating memories: molecular mechanisms of CRISPR adaptation.Trends in biochemical sciences · 2022Review
- Distribution and phasing of sequence motifs that facilitate CRISPR adaptation.Current biology : CB · 2021Article
- Robust direct digital-to-biological data storage in living cells.Nature chemical biology · 2021Article
- CRISPR Arrays Away fromThe CRISPR journal · 2020Article
- Detection of CRISPR adaptation.Biochemical Society transactions · 2020Review
- The Clustered Regularly Interspaced Short Palindromic Repeat System and Argonaute: An Emerging Bacterial Immunity System for Defense Against Natural Transformation?Frontiers in microbiology · 2020Review
- CRISPR repeat sequences and relative spacing specify DNA integration by Pyrococcus furiosus Cas1 and Cas2.Nucleic acids research · 2019Article
- Spermidine strongly increases the fidelity ofThe Journal of biological chemistry · 2019Article
- Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems.FEMS microbiology letters · 2019Article
- Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems.RNA biology · 2019Article
- A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.Molecular cell · 2019Article
- Selective Maintenance of Multiple CRISPR Arrays Across Prokaryotes.The CRISPR journal · 2018Article
- DNA-based memory devices for recording cellular events.Nature reviews. Genetics · 2018Review
- CRISPRs from scratch.Nature microbiology · 2018Article
- Bacterial physiology: Spacers go off-site.Nature reviews. Microbiology · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The adaptation phase of CRISPR-Cas immunity depends on the precise integration of short segments of foreign DNA (spacers) into a specific genomic location within the CRISPR locus by the Cas1-Cas2 integration complex. Although off-target spacer integration outside of canonical CRISPR arrays has been described in vitro, no evidence of non-specific integration activity has been found in vivo. Here, we show that non-canonical off-target integrations can occur within bacterial chromosomes at locations that resemble the native CRISPR locus by characterizing hundreds of off-target integration locations within Escherichia coli. Considering whether such promiscuous Cas1-Cas2 activity could have an evolutionary role through the genesis of neo-CRISPR loci, we combed existing CRISPR databases and available genomes for evidence of off-target integration activity. This search uncovered several putative instances of naturally occurring off-target spacer integration events within the genomes of Yersinia pestis and Sulfolobus islandicus. These results are important in understanding alternative routes to CRISPR array genesis and evolution, as well as in the use of spacer acquisition in technological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.