ArticleScientific reports2018
Using DNA origami nanorulers as traceable distance measurement standards and nanoscopic benchmark structures.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Nanorulers for Super-Resolution Microscopy.Chembiochem : a European journal of chemical biology · 2026Review
- Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single-molecule photobleaching step counting and spatio-temporal colocalization.Biomedical optics express · 2026Article
- FISH-Dist: An Automated Pipeline for 3D Genomic Spatial Distance Quantification in FISH Imaging.Bioengineering (Basel, Switzerland) · 2026Article
- Uncovering Design and Assembly Rules for mRNA-DNA Origami.Nano letters · 2026Article
- Plasmonic Nano-bolas Hunt DNA Targets.ACS nanoscience Au · 2026Article
- A comparison of super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium.Journal of microscopy · 2026Article
- Integrating the Contrasting Perspectives Between the Constrained Disorder Principle and Deterministic Optical Nanoscopy: Enhancing Information Extraction from Imaging of Complex Systems.Bioengineering (Basel, Switzerland) · 2026Article
- One-step nanoscale expansion microscopy reveals individual protein shapes.Nature biotechnology · 2025Article
- Quality control maps: Real-time quantitative control of single-molecule localization microscopy data.Biophysical journal · 2025Article
- Microtubules as a versatile reference standard for expansion microscopy.Communications biology · 2025Article
- A DNA-Based Plasmonic Nano-Ruler.International journal of molecular sciences · 2025Article
- Fully addressable designer superstructures assembled from one single modular DNA origami.Nature communications · 2025Article
- DNA Hybridization Kinetics Observed at the Single-Molecule Level Using Graphene Near-Field Effects.The journal of physical chemistry. A · 2024Article
- Traceable localization enables accurate integration of quantum emitters and photonic structures with high yield.Optica quantum · 2024Article
- Signal Amplification in Electrochemical DNA Biosensors Using Target-Capturing DNA Origami Tiles.ACS sensors · 2023Article
- Lab-on-a-DNA origami: nanoengineered single-molecule platforms.Chemical communications (Cambridge, England) · 2023Review
- Biomolecule-Based Optical Metamaterials: Design and Applications.Biosensors · 2022Review
- Water-in-Water Droplets Selectively Uptake Self-Assembled DNA Nano/Microstructures: a Versatile Method for Purification in DNA Nanotechnology.Chembiochem : a European journal of chemical biology · 2022Article
- Can super-resolution microscopy become a standard characterization technique for materials chemistry?Chemical science · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, DNA origami nanorulers for superresolution (SR) fluorescence microscopy have been developed from fundamental proof-of-principle experiments to commercially available test structures. The self-assembled nanostructures allow placing a defined number of fluorescent dye molecules in defined geometries in the nanometer range. Besides the unprecedented control over matter on the nanoscale, robust DNA origami nanorulers are reproducibly obtained in high yields. The distances between their fluorescent marks can be easily analysed yielding intermark distance histograms from many identical structures. Thus, DNA origami nanorulers have become excellent reference and training structures for superresolution microscopy. In this work, we go one step further and develop a calibration process for the measured distances between the fluorescent marks on DNA origami nanorulers. The superresolution technique DNA-PAINT is used to achieve nanometrological traceability of nanoruler distances following the guide to the expression of uncertainty in measurement (GUM). We further show two examples how these nanorulers are used to evaluate the performance of TIRF microscopes that are capable of single-molecule localization microscopy (SMLM).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.