ArticleNature communications2018
Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Clathrin is an Intrinsic Driver of Membrane Fission.bioRxiv : the preprint server for biology · 2026Article
- Endocytic Adaptor Proteins in Vascular Diseases: Critical Role of Intracellular Trafficking in Regulating Pathobiology of Vascular Disorders.Cardiology discovery · 2026Review
- Vesiculation pathways in clathrin-mediated endocytosis.bioRxiv : the preprint server for biology · 2025Article
- Dynamic early recruitment of GAK-Hsc70 regulates coated pit maturation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells.Biophysical journal · 2025Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Antibody surface mobility amplifies FcγR signaling via Arp2/3 during phagocytosis.Biophysical journal · 2024Article
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells.bioRxiv : the preprint server for biology · 2024Article
- Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging.Journal of imaging · 2023Review
- Clathrin coats partially preassemble and subsequently bend during endocytosis.The Journal of cell biology · 2023Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells.Nature communications · 2023Article
- Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation.Bioscience reports · 2022Review
- Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges.Nature communications · 2022Article
- Genetic pain loss disorders.Nature reviews. Disease primers · 2022Review
- Load adaptation by endocytic actin networks.Molecular biology of the cell · 2022Article
- Coarse-Grained Simulations Suggest Potential Competing Roles of Phosphoinositides and Amphipathic Helix Structures in Membrane Curvature Sensing of the AP180 N-Terminal Homology Domain.The journal of physical chemistry. B · 2022Article
- Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.Nature communications · 2022Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Clathrin-mediated endocytosis (CME) internalizes plasma membrane by reshaping small regions of the cell surface into spherical vesicles. The key mechanistic question of how coat assembly produces membrane curvature has been studied with molecular and cellular structural biology approaches, without direct visualization of the process in living cells; resulting in two competing models for membrane bending. Here we use polarized total internal reflection fluorescence microscopy (pol-TIRF) combined with electron, atomic force, and super-resolution optical microscopy to measure membrane curvature during CME. Surprisingly, coat assembly accommodates membrane bending concurrent with or after the assembly of the clathrin lattice. Once curvature began, CME proceeded to scission with robust timing. Four color pol-TIRF showed that CALM accumulated at high levels during membrane bending, implicating its auxiliary role in curvature generation. We conclude that clathrin-coat assembly is versatile and that multiple membrane-bending trajectories likely reflect the energetics of coat assembly relative to competing forces.
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