Evidence map›Paper›PMID 29378560›Full record

ArticleBMC complementary and alternative medicine2018

Sheng-ji Hua-yu formula promotes diabetic wound healing of re-epithelization via Activin/Follistatin regulation.

Le Kuai, Jing-Ting Zhang, Yu Deng, Shun Xu, Xun-Zhe Xu, Min-Feng Wu, Dong-Jie Guo, Yu Chen, Ren-Jie Wu, Xing-Qiang Zhao and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary and alternative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 43 citations in OpenAlex.

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  18. Application of a Cloud Model-Set Pair Analysis in Efficacy Assessment for Diabetic Ulcers.Evidence-based complementary and alternative medicine : eCAM · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Le KuaiDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Jing-Ting ZhangPharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Yu DengSchool of Medicine, Chengdu University, Chengdu, 610106, China.
Shun XuDepartment of Burns and Plastic Surgery, The Seventh People's Hospital of Integrated Traditional Chinese and Western Medicine, affiliated with Shanghai University of Traditional Chinese Medicine, Shanghai, 200137, China.
Xun-Zhe XuDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Min-Feng WuDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Dong-Jie GuoDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Yu ChenDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Ren-Jie WuDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Xing-Qiang ZhaoDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Hua NianPharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China.
Bin LiDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China. 18930568129@163.com.
Fu-Lun LiDepartment of Dermatology of Yueyang Hospital, Shanghai University of TCM, Shanghai, 200437, China. drlifulun@163.com.
Shanghai University of Traditional Chinese Medicine · CNYueyang Hospital · CNShanghai University · CNChengdu University · CN

Funding

Grant from Shanghai University TCM 201610268065Grant from Shanghai University TCM 2016KCS09National Science Foundation (NSFC) of China 81373648National Science Foundation (NSFC) of China 81602652National Science Foundation (NSFC) of China 81673866National Science Foundation (NSFC) of China J1103607Shanghai Science and Technology Committee 16411955000The project of Shanghai Health Bureau project ZY3-CCCX-3-3033
6 · The paper itself

Abstract

backgroundSheng-ji Hua-yu(SJHY) formula is one of the most useful Traditional Chinese medicine (TCM) in the treatment of the delayed diabetic wound. However, elucidating the related molecular biological mechanism of how the SJHY Formula affects excessive inflammation in the process of re-epithelialization of diabetic wound healing is a task urgently needed to be fulfilled. The objectives of this study is to evaluate the effect of antagonisic expression of pro-/anti-inflammatory factors on transforming growth factor-β(TGF-β) superfamily (activin and follistatin) in the process of re-epithelialization of diabetic wound healing in vivo, and to characterize the involvement of the activin/follistatin protein expression regulation, phospho-Smad (pSmad2), and Nuclear factor kappa B p50 (NF-kB) p50 in the diabetic wound healing effects of SJHY formula.

methodsSJHY Formula was prepared by pharmaceutical preparation room of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine. Diabetic wound healing activity was evaluated by circular excision wound models. Wound healing activity was examined by macroscopic evaluation. Activin/follistatin expression regulation, protein expression of pSmad2 and NF-kB p50 in skin tissue of wounds were analyzed by Real Time PCR, Western blot, immunohistochemistry and hematoxylin and eosin (H&E) staining.

resultsMacroscopic evaluation analysis showed that wound healing of diabetic mice was delayed, and SJHY Formula accelerated wound healing time of diabetic mice. Real Time PCR analysis showed higher mRNA expression of activin/follistatin in diabetic delayed wound versus the wound in normal mice. Western Blot immunoassay analysis showed reduction of activin/follistatin proteins levels by SJHY Formula treatment 15 days after injury. Immunohistochemistry investigated the reduction of pSmad2 and NF-kB p50 nuclear staining in the epidermis of diabetic SJHY versus diabetic control mice on day 15 after wounding. H&E staining revealed that SJHY Formula accelerated re-epithelialization of diabetic wound healing.

conclusionThe present study found that diabetic delayed wound healing time is closely related to the high expression level of activin/follistatin, which leads to excessive inflammation in the process of re-epithelization. SJHY Formula accelerates re-epithelialization and healing time of diabetic wounds through decreasing the high expression of activin/follistatin.

Indexed as

ActivinsAnimalsDiabetes Mellitus, ExperimentalDrugs, Chinese HerbalFemaleFollistatinMiceMice, Inbred C57BLRe-EpithelializationUlcerActivinsDrugs, Chinese HerbalFollistatinActivinChinese herbalDiabetic ulcerFollistatinInflammationRe-epithelizationWound healing

Identifiers

PMID29378560
PMCPMC5789635
OpenAlexW2792943924

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.