Evidence map›Paper›PMID 29378248›Full record

ArticleNitric oxide : biology and chemistry2018

Effect of dietary nitrate levels on nitrate fluxes in rat skeletal muscle and liver.

Cameron N Gilliard, Jeff K Lam, Katelyn S Cassel, Ji Won Park, Alan N Schechter, Barbora Piknova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nitric oxide : biology and chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 4 pooled it
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 4 syntheses or guidelines pooled it, 60 citations in OpenAlex.

  1. Pooled it
  2. The Effects of Dietary Nitrate Supplementation on Explosive Exercise Performance: A Systematic Review.International journal of environmental research and public health · 2022
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  13. Effect of pre-exercise dietary nitrate on skeletal muscle blood flow in a rat model of pulmonary hypertension.American journal of physiology. Regulatory, integrative and comparative physiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Cameron N GilliardMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States; Penn State Health Milton S. Hershey Medical Center, Department of Anesthesiology, Hershey, PA, United States.
Jeff K LamMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States; Icahn School of Medicine, Mt. Sinai, New York, NY, United States.
Katelyn S CasselMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States.
Ji Won ParkMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States.
Alan N SchechterMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States.
Barbora PiknovaMolecular Medicine Branch, NIDDK, NIH, Bethesda, MD, United States. Electronic address: piknovab@mail.nih.gov.
National Institutes of Health · USIcahn School of Medicine at Mount Sinai · USPenn State Milton S. Hershey Medical Center · US

Funding

Clinical Applications of Nitrite and NitrateZIADK025104 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI SCHECHTER, ALAN · 2009 to 2025
$10.4M
Intramural NIH HHS Z99 DK999999
6 · The paper itself

Abstract

Rodent skeletal muscle has high levels of nitrate ions and this endogenous nitrate reservoir can supply nitrite/nitric oxide (NO) for functional hyperemia and/or for other physiological processes in muscle during exercise. Mice with a NOS1 knockout have markedly reduced muscle nitrate levels, suggesting NO production by NOS and its reaction with oxymyoglobin as a source of nitrate. However, oxygen levels are normally low in most internal organs, which raises the possibility that nitrate-derived NO pathway is physiologically important even at "normoxia", and muscle nitrate reservoir is the main endogenous NO backup when exogeneous (dietary) nitrate intake is low. Using dietary nitrate manipulations, we explore the importance of diet for maintaining and renewal of muscle nitrate reservoir and its levels in other tissues. We found that skeletal muscle nitrate is extensively used when nitrate in diet is low. One week of nitrate starvation leads to dramatic nitrate depletion in skeletal muscle and a substantial decrease in liver. Nitrate depleted from skeletal muscle during starvation is quickly recovered from new dietary sources, with an unexpected significant "overload" compared with animals not subjected to nitrate starvation. Our results suggest the importance of dietary nitrate for nitrate reserves in muscle and in other tissues, when compared with endogenous NOS-derived sources. This requires an active transport mechanism for sequestering nitrate into cells, stimulated by lack of dietary nitrate or other enzymatic changes. These results confirm the hypothesis that muscle is a major storage site for nitrate in mammals.

Indexed as

AnimalsDietLiverMuscle, SkeletalNitratesNitritesRats, WistarNitratesNitritesDietLiverNitrateNitriteSkeletal muscle

Identifiers

PMID29378248
PMCPMC5860979
OpenAlexW2794369712

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.