ArticlePloS one2018
Recellularization of rat liver: An in vitro model for assessing human drug metabolism and liver biology.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- A Review on Bioengineering the Bovine Mammary Gland: The Role of the Extracellular Matrix and Reconstruction Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- Decellularized Liver Matrices for Expanding the Donor Pool-An Evaluation of Existing Protocols and Future Trends.Biomolecules · 2025Review
- Two Decades of Advances and Limitations in Organ Recellularization.Current issues in molecular biology · 2024Review
- Role and potential therapeutic strategies of matrix mechanics for optimizing tumor radiotherapy.Mechanobiology in medicine · 2024Review
- Organoids and Their Research Progress in Plastic and Reconstructive Surgery.Aesthetic plastic surgery · 2023Review
- Human induced pluripotent stem cell-derived liver-on-a-chip for studying drug metabolism: the challenge of the cytochrome P450 family.Frontiers in pharmacology · 2023Review
- Novel organoid model in drug screening: Past, present, and future.Liver research (Beijing, China) · 2021Review
- A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs.Nanomaterials (Basel, Switzerland) · 2021Article
- Sustained perfusion of revascularized bioengineered livers heterotopically transplanted into immunosuppressed pigs.Nature biomedical engineering · 2020Article
- Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering.Frontiers in bioengineering and biotechnology · 2020Article
- Current progress in hepatic tissue regeneration by tissue engineering.Journal of translational medicine · 2019Review
- A human-on-a-chip approach to tackling rare diseases.Drug discovery today · 2019Review
- Human-animal chimeras for autologous organ transplantation: technological advances and future perspectives.Annals of translational medicine · 2019Review
- Liver Bioengineering: Promise, Pitfalls, and Hurdles to Overcome.Current transplantation reports · 2019Article
- Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold.Stem cells international · 2019Review
- Scaffold-mediated liver regeneration: A comprehensive exploration of current advances.Journal of tissue engineeringReview
- Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liver-like organoids that recapitulate the complex functions of the whole liver by combining cells, scaffolds, and mechanical or chemical cues are becoming important models for studying liver biology and drug metabolism. The advantages of growing cells in three-dimensional constructs include enhanced cell-cell and cell-extracellular matrix interactions and preserved cellular phenotype including, prevention of de-differentiation. In the current study, biomimetic liver constructs were made via perfusion decellularization of rat liver, with the goal of maintaining the native composition and structure of the extracellular matrix. We optimized our decellularization process to produce liver scaffolds in which immunogenic residual DNA was removed but glycosaminoglycans were maintained. When the constructs were recellularized with rat or human liver cells, the cells remained viable, capable of proliferation, and functional for 28 days. Specifically, the cells continued to express cytochrome P450 genes and maintained their ability to metabolize a model drug, midazolam. Microarray analysis showed an upregulation of genes involved in liver regeneration and fibrosis. In conclusion, these liver constructs have the potential to be used as test beds for studying liver biology and drug metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.