ArticleCellular microbiology2018
Association of Vibrio cholerae 569B outer membrane vesicles with host cells occurs in a GM1-independent manner.
Article in Cellular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Outer membrane vesicles inMicrobial cell (Graz, Austria) · 2026Article
- Heterogeneity of Size and Toxin Distribution inToxins · 2024Article
- Characterization of the Inflammatory Response Evoked by Bacterial Membrane Vesicles in Intestinal Cells Reveals an RIPK2-Dependent Activation by Enterotoxigenic Escherichia coli Vesicles.Microbiology spectrum · 2023Article
- Composition and functions of bacterial membrane vesicles.Nature reviews. Microbiology · 2023Review
- The Two Faces of Bacterial Membrane Vesicles: Pathophysiological Roles and Therapeutic Opportunities.Antibiotics (Basel, Switzerland) · 2023Review
- Emerging role of microbiota derived outer membrane vesicles to preventive, therapeutic and diagnostic proposes.Infectious agents and cancer · 2023Review
- Review
- T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer.The ISME journal · 2022Article
- Review
- Article
- Article
- Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles.Frontiers in immunology · 2021Review
- Aggregatibacter actinomycetemcomitans leukotoxin: From mechanism to targeted anti-toxin therapeutics.Molecular oral microbiology · 2020Review
- The Uptake, Trafficking, and Biodistribution ofFrontiers in microbiology · 2020Article
- Cholera Toxin Encapsulated within SeveralToxins · 2019Article
- Inhibition of bacterial toxin recognition of membrane components as an anti-virulence strategy.Journal of biological engineering · 2019Review
- Outer Membrane Vesicle-Host Cell Interactions.Microbiology spectrum · 2019Article
- Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based.Diagnostic pathology · 2018Review
- Article
- Insights into flagellar function and mechanism from the squid-vibrio symbiosis.NPJ biofilms and microbiomesReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The primary virulence factor of Vibrio cholerae, cholera toxin (CT), initiates a pathway in epithelial cells that leads to the severe diarrhoea characteristic of cholera. Secreted CT binds to GM1 on the surface of host cells to facilitate internalisation. Many bacterial toxins, including CT, have been shown to be additionally delivered via outer membrane vesicles (OMVs). A fraction of the closely related heat labile toxin produced by enterotoxigenic Escherichia coli has been demonstrated to reside on the surface of OMVs, where it binds GM1 to facilitate OMV internalisation by host cells. In this work, we investigated whether OMV-associated CT is likewise trafficked to host cells in a GM1-dependent mechanism. We demonstrated that a majority of CT is secreted in its OMV-associated form and is located exclusively inside the vesicle. Therefore, the toxin is unable to bind GM1 on the host cell surface, and the OMVs are trafficked to the host cells in a GM1-independent mechanism. These findings point to a secondary, noncompeting mechanism for secretion and delivery of CT, beyond its well-studied secretion via a Type II secretion system and underscore the importance of focusing future studies on understanding this GM1-independent delivery mechanism to fully understand Vibrio cholerae pathogenesis.
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