ArticleJournal of fungi (Basel, Switzerland)2016
Colony-PCR Is a Rapid Method for DNA Amplification of Hyphomycetes.
Article in Journal of fungi (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
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- Scalable genotyping of microbial colonies.Microbial genomics · 2025Article
- Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass-Tips and Considerations.Bio-protocol · 2023Article
- Rapid Monitoring of Viable Genetically ModifiedMicroorganisms · 2023Article
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- Multiplex PCR Identification ofFoods (Basel, Switzerland) · 2022Article
- Petri-plate, bacteria, and laser optical scattering sensor.Frontiers in cellular and infection microbiology · 2022Review
- Many foliar endophytic fungi of Quercus gambelii are capable of psychrotolerant saprotrophic growth.PloS one · 2022Article
- Application of direct PCR for phylogenetic analysis ofFrontiers in plant science · 2022Article
- High-Throughput Volatilome Fingerprint Using PTR-ToF-MS Shows Species-Specific Patterns inJournal of fungi (Basel, Switzerland) · 2021Article
- Habitat, Snow-Cover and Soil pH, Affect the Distribution and Diversity of Mortierellaceae Species and Their Associations to Bacteria.Frontiers in microbiology · 2021Article
- Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically TransformedLife (Basel, Switzerland) · 2020Article
- Emerging from the ice-fungal communities are diverse and dynamic in earliest soil developmental stages of a receding glacier.Environmental microbiology · 2019Article
- How to resolve cryptic species of polypores: an example inIMA fungus · 2019Article
- A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms.Mycobiology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal pure cultures identified with both classical morphological methods and through barcoding sequences are a basic requirement for reliable reference sequences in public databases. Improved techniques for an accelerated DNA barcode reference library construction will result in considerably improved sequence databases covering a wider taxonomic range. Fast, cheap, and reliable methods for obtaining DNA sequences from fungal isolates are, therefore, a valuable tool for the scientific community. Direct colony PCR was already successfully established for yeasts, but has not been evaluated for a wide range of anamorphic soil fungi up to now, and a direct amplification protocol for hyphomycetes without tissue pre-treatment has not been published so far. Here, we present a colony PCR technique directly from fungal hyphae without previous DNA extraction or other prior manipulation. Seven hundred eighty-eight fungal strains from 48 genera were tested with a success rate of 86%. PCR success varied considerably: DNA of fungi belonging to the genera
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