ArticleFrontiers in microbiology2017
Leaf-FISH: Microscale Imaging of Bacterial Taxa on Phyllosphere.
Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Tree tissues and species traits modulate the microbial methane-cycling communities of the tree phyllosphere.Scientific reports · 2026Article
- Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere.BMC biology · 2024Review
- From microbiome composition to functional engineering, one step at a time.Microbiology and molecular biology reviews : MMBR · 2023Review
- Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria.The New phytologist · 2023Article
- Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ.Nature communications · 2022Article
- Changes of Endophytic Bacterial Community in Mature Leaves ofPlants (Basel, Switzerland) · 2022Article
- microbeSEG: A deep learning software tool with OMERO data management for efficient and accurate cell segmentation.PloS one · 2022Article
- Environmental connectivity controls diversity in soil microbial communities.Communications biology · 2021Article
- Two-way microscale interactions between immigrant bacteria and plant leaf microbiota as revealed by live imaging.The ISME journal · 2021Article
- Shaping the leaf microbiota: plant-microbe-microbe interactions.Journal of experimental botany · 2021Article
- FISH Variants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Microbiological and Nutritional Analysis of Lettuce Crops Grown on the International Space Station.Frontiers in plant science · 2020Article
- Article
- Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology.Scientific reports · 2019Article
- Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular methods for microbial community characterization have uncovered environmental and plant-associated factors shaping phyllosphere communities. Variables undetectable using bulk methods can play an important role in shaping plant-microbe interactions. Microscale analysis of bacterial dynamics in the phyllosphere requires imaging techniques specially adapted to the high autoflouresence and 3-D structure of the leaf surface. We present an easily-transferable method (Leaf-FISH) to generate high-resolution tridimensional images of leaf surfaces that allows simultaneous visualization of multiple bacterial taxa in a structurally informed context, using taxon-specific fluorescently labeled oligonucleotide probes. Using a combination of leaf pretreatments coupled with spectral imaging confocal microscopy, we demonstrate the successful imaging bacterial taxa at the genus level on cuticular and subcuticular leaf areas. Our results confirm that different bacterial species, including closely related isolates, colonize distinct microhabitats in the leaf. We demonstrate that highly related
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.