ArticleMicrobial cell factories2018
Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- The R plasmid pRAS2 in the fish pathogenFrontiers in microbiology · 2026Article
- Plasmid-mediated horizontal gene mobilisation: Insights from two lactococcal conjugative plasmids.Microbial biotechnology · 2024Article
- A New Tool for the Flexible Genetic Manipulation ofBio-protocol · 2022Article
- A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation.Microbial cell factories · 2022Article
- Assessment ofMicroorganisms · 2021Article
- Delineation of ISFrontiers in microbiology · 2021Article
- Co-Inoculation ofMicroorganisms · 2020Article
- Trans-Kingdom Conjugation within Solid Media fromInternational journal of molecular sciences · 2019Article
- Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms.Microbial biotechnology · 2019Review
- A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus.Microbial cell factories · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid.
resultsIn this study, the oriT sequence of pLS20cat (oriT
conclusionsThe chromosomal DNA mobilization mediated by pLS20catΔoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome.
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