ArticleScientific reports2018
Three-dimensional histochemistry and imaging of human gingiva.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Prevalence and practices of immunofluorescent cell image processing: a systematic review.Frontiers in cellular neuroscience · 2023Pooled it
- Biofabrication Strategies for Oral Soft Tissue Regeneration.Advanced healthcare materials · 2024Review
- Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice.Journal of diabetes research · 2023Article
- Human gingival mesenchymal stem cells retain their growth and immunomodulatory characteristics independent of donor age.Science advances · 2022Article
- Identification of venular capillary remodelling: a possible link to the development of periodontitis?Journal of periodontal & implant science · 2022Article
- The New Era of Three-Dimensional Histoarchitecture of the Human Endometrium.Journal of personalized medicine · 2021Review
- Optimizing tissue clearing and imaging methods for human brain tissue.The Journal of international medical research · 2021Article
- Light sheet fluorescence microscopy as a new method for unbiased three-dimensional analysis of vascular injury.Cardiovascular research · 2021Article
- Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Poor perfusion of the microvasculature in peritoneal metastases of ovarian cancer.Clinical & experimental metastasis · 2020Observational
- Light Sheet Microscopy Using FITC-Albumin Followed by Immunohistochemistry of the Same Rehydrated Brains Reveals Ischemic Brain Injury and Early Microvascular Remodeling.Frontiers in cellular neuroscience · 2020Article
- Similarities Between Stem Cell Niches in Glioblastoma and Bone Marrow: Rays of Hope for Novel Treatment Strategies.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Elevated ATF4 Expression in Odontogenic Keratocysts Epithelia: Potential Involvement in Tissue Hypoxia and Stromal M2 Macrophage Infiltration.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Light sheet microscopy for histopathology applications.Biomedical engineering letters · 2019Review
- Article
- Fidelity test for through-focus or volumetric type of optical imaging methods.Optics express · 2018Article
- Through-focus or volumetric type of optical imaging methods: a review.Journal of biomedical optics · 2018Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, 3D histochemistry and imaging methodology is described for human gingiva to analyze its vascular network. Fifteen human gingiva samples without signs of inflammation were cleared using a mixture of 2-parts benzyl benzoate and 1-part benzyl alcohol (BABB), after being immunofluorescently stained for CD31, marker of endothelial cells to visualize blood vessels in combination with fluorescent DNA dyes. Samples were imaged in 3D with the use of confocal microscopy and light-sheet microscopy and image processing. BABB clearing caused limited tissue shrinkage 13 ± 7% as surface area and 24 ± 1% as volume. Fluorescence remained intact in BABB-cleared gingiva samples and light-sheet microscopy was an excellent tool to image gingivae whereas confocal microscopy was not. Histochemistry on cryostat sections of gingiva samples after 3D imaging validated structures visualized in 3D. Three-dimensional images showed the vascular network in the stroma of gingiva with one capillary loop in each stromal papilla invading into the epithelium. The capillary loops were tortuous with structural irregularities that were not apparent in 2D images. It is concluded that 3D histochemistry and imaging methodology described here is a promising novel approach to study structural aspects of human gingiva in health and disease.
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