Evidence map›Paper›PMID 29373460›Full record

ArticleThe journal of trauma and acute care surgery2018

Early low-anticoagulant desulfated heparin after traumatic brain injury: Reduced brain edema and leukocyte mobilization is associated with improved watermaze learning ability weeks after injury.

Katsuhiro Nagata, Yujin Suto, John Cognetti, Kevin D Browne, Kenichiro Kumasaka, Victoria E Johnson, Lewis Kaplan, Joshua Marks, Douglas H Smith, Jose L Pascual

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of trauma and acute care surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Katsuhiro NagataFrom the Division of Traumatology, Surgical Critical Care & Emergency Surgery (K.N., Y.S., L.K., J.L.P.) and Department of Neurosurgery Center for Brain Injury, and Repair (K.N., Y.S., J.C., K.D.B., V.E.J., D.H.S., J.L.P.) University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania; Department of Emergency and Critical Care Medicine (K.N., K.K.), Tokyo Medical University Hachioji Medical Center, Tokyo, Japan; and Sidney Kimmel Medical College at Thomas Jefferson University (J.M.), Philadelphia, Pennsylvania.
Yujin Suto
John Cognetti
Kevin D Browne
Kenichiro Kumasaka
Victoria E Johnson
Lewis Kaplan
Joshua Marks
Douglas H Smith
Jose L Pascual
Norwegian Womens Public Health Association · NO

Funding

Neuropathological Characterization of 'CTE'R01NS094003 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI Douglas Hamilton Smith, William Stewart · 2016 to 2026
$5.1M
Detection and Mechanisms of Mild Traumatic Brain InjuryR01NS092398 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI SMITH, DOUGLAS HAMILTON · 2015 to 2019
$1.9M
NINDS NIH HHS R01 NS092398NINDS NIH HHS R01 NS094003
6 · The paper itself

Abstract

backgroundUnfractionated heparin administered immediately after traumatic brain injury (TBI) reduces brain leukocyte (LEU) accumulation, and enhances early cognitive recovery, but may increase bleeding after injury. It is unknown how non-anticoagulant heparins, such as 2,3-O desulfated heparin (ODSH), impact post-TBI cerebral inflammation and long-term recovery. We hypothesized that ODSH after TBI reduces LEU-mediated brain inflammation and improves long-term neurologic recovery.

methodsCD1 male mice (n = 66) underwent either TBI (controlled cortical impact [CCI]) or sham craniotomy. 2,3-O desulfated heparin (25 mg/kg [25ODSH] or 50 mg/kg [50ODSH]) or saline was administered for 48 hours after TBI in 46 animals. At 48 hours, intravital microscopy visualized rolling LEUs and fluorescent albumin leakage in the pial circulation, and the Garcia Neurologic Test assessed neurologic function. Brain edema (wet/dry ratio) was evaluated post mortem. In a separate group of animals (n = 20), learning/memory ability (% time swimming in the Probe platform quadrant) was assessed by the Morris Water Maze 17 days after TBI. Analysis of variance with Bonferroni correction determined significance (p < 0.05).

resultsCompared with CCI (LEU rolling: 32.3 ± 13.7 LEUs/100 μm per minute, cerebrovascular albumin leakage: 57.4 ± 5.6%), both ODSH doses reduced post-TBI pial LEU rolling (25ODSH: 18.5 ± 9.2 LEUs/100 μm per minute, p = 0.036; 50ODSH: 7.8 ± 3.9 LEUs/100 μm per minute, p < 0.001) and cerebrovascular albumin leakage (25ODSH: 37.9 ± 11.7%, p = 0.001, 50ODSH: 32.3 ± 8.7%, p < 0.001). 50ODSH also reduced injured cerebral hemisphere edema (77.7 ± 0.4%) vs. CCI (78.7 ± 0.4 %, p = 0.003). Compared with CCI, both ODSH doses improved Garcia Neurologic Test at 48 hours. Learning/memory ability (% time swimming in target quadrant) was lowest in CCI (5.9 ± 6.4%) and significantly improved in the 25ODSH group (27.5 ± 8.2%, p = 0.025).

conclusion2,3-O desulfated heparin after TBI reduces cerebral LEU recruitment, microvascular permeability and edema. 2,3-O desulfated heparin may also improve acute neurologic recovery leading to improved learning/memory ability weeks after injury.

Indexed as

AnimalsBrain EdemaBrain Injuries, TraumaticCapillary PermeabilityCognitionDisease Models, AnimalFollow-Up StudiesHeparinLeukocyte RollingMaleMaze LearningMiceTime FactorsHeparinheparin, O-desulfated

Identifiers

PMID29373460
PMCPMC9380841
OpenAlexW2790237673

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.