Evidence map›Paper›PMID 29372514›Full record

ArticleJournal of applied genetics2018

Gene cloning system for sulfonamide-mineralizing Microbacterium sp. strain BR1.

I Ostash, B Kolvenbach, P F-X Corvini, V Fedorenko, B Ostash, Danuta Cichocka

Abstract read
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In one paragraph

Article in Journal of applied genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

I OstashDepartment of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine.
B KolvenbachInstitute for Ecopreneurship School of Life Sciences University of Applied Sciences and Arts Northwestern Switzerland - FHNW, Gründenstrasse 40, 4132, Muttenz, Switzerland.
P F-X CorviniInstitute for Ecopreneurship School of Life Sciences University of Applied Sciences and Arts Northwestern Switzerland - FHNW, Gründenstrasse 40, 4132, Muttenz, Switzerland.
V FedorenkoDepartment of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine.
B OstashDepartment of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine.
Danuta CichockaInstitute for Ecopreneurship School of Life Sciences University of Applied Sciences and Arts Northwestern Switzerland - FHNW, Gründenstrasse 40, 4132, Muttenz, Switzerland. dankacich@gmail.com.
FHNW University of Applied Sciences and Arts · CHLviv University · UA

Funding

Ministry of Education and Science of Ukraine Bg-46FSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung IZK0Z3_154372Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (CH) 407240_167103 and 310030L_160332
6 · The paper itself

Abstract

The wide application of sulfonamide (SA) antibiotics in human and veterinary medicine contributes to the accumulation of these antibiotics in the environment and the corresponding onset of antibiotic resistance among bacteria. Microbacterium sp. BR1 is capable of mineralizing sulfamethoxazole and other SAs via a novel mechanism. The genetic basis of SA elimination by BR1 remains unknown. Development of an efficient plasmid transfer protocol for Microbacterium sp. BR1 is highly desirable, as it would open the door to genetic analysis and manipulation of its genome. Here we report that intergeneric Escherichia coli-Microbacterium spp. BR1 conjugation is an efficient way to introduce various plasmids into BR1. The generated transconjugants were stable in the presence of antibiotics and the plasmids showed no signs of rearrangements. Nevertheless, the plasmids were rapidly lost in the absence of selection. We also show that the cumate-inducible beta-glucuronidase reporter gene functions in BR1 and is strictly regulated. Our results set the working ground for further genetic manipulations of BR1, such as the overexpression of sulfonamide degradation genes or the selection of strong microbacterial promoters.

Indexed as

Cloning, MolecularConjugation, GeneticActinomycetalesEscherichia coliGenetic VectorsPlasmidsSulfonamidesSulfonamidesGene cloningIntergeneric conjugationMicrobacteriumSulfamethoxazole

Identifiers

PMID29372514
OpenAlexW2788709584

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.