ArticleJournal of animal science and biotechnology2018
Optimization of culture conditions for short-term maintenance, proliferation, and colony formation of porcine gonocytes.
Article in Journal of animal science and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Optimized Recovery of Immature Germ Cells after Prepubertal Testicular Tissue Digestion and Multi-Step Differential Plating: A Step towards Fertility Restoration with Cancer-Cell-Contaminated Tissue.International journal of molecular sciences · 2023Article
- Brief exposure of neonatal testis cells to EGF or GDNF alters the regenerated tissue.Reproduction & fertility · 2022Article
- Neonatal Porcine Germ Cells Dedifferentiate and Display Osteogenic and Pluripotency Properties.Cells · 2021Article
- Article
- Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility.Cells · 2020Review
- Manipulation of spermatogonial stem cells in livestock species.Journal of animal science and biotechnology · 2019Review
- Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak.Journal of animal science and biotechnology · 2019Article
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Authors and funding
2 authors.
Funding
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Abstract
backgroundGonocytes give rise to spermatogonial stem cells, and thereby play an essential role in establishing spermatogenesis. Optimized culture conditions for gonocytes provide an opportunity for their study and in vitro manipulation for potential application in reproductive technologies. Using six experiments in a step-wise design, we examined the effects of several culture conditions on the maintenance, proliferation, and colony formation of porcine gonocytes. Testis cells from neonatal piglets were cultured for 7 d in DMEM supplemented with 10% fetal bovine serum. The examined culture conditions included using different cell seeding densities, gonocyte proportions, incubation temperatures, sampling strategies, and medium changing regimens.
resultsConfluency of cells was optimal (>90% by ~6 d) when 3.0 × 10
conclusionWe found that gonocytes require the presence of a critical minimum number of somatic cells for settlement, and can proliferate and form growing colonies even in a basic medium. Large numbers of viable gonocytes remain floating in the medium for several days. The optimized culture conditions in the present study included seeding with 3.0 × 10
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