ArticleBiotechnology for biofuels2018
Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9.
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 129 citations in OpenAlex.
- Plant Biomass Depolymerization by Oxidoreductases: Structural Characteristics, Synergy Mechanism, and Evolutionary Adaptation.Journal of agricultural and food chemistry · 2026Review
- Characterization of an AA9 LPMO from Fusarium oxysporum and its role in cellulose hydrolysis and functionalization.Biotechnology for biofuels and bioproducts · 2026Article
- Molecular identification of ligninolytic fungi from West Sumatra, Indonesia, using the Internal Transcribed Spacer (ITS) region.Journal of advanced veterinary and animal research · 2026Article
- Genomic, Proteomic, and Biochemical Study ofJournal of agricultural and food chemistry · 2025Article
- Saprotrophic Wood Decay Ability and Plant Cell Wall Degrading Enzyme System of the White Rot FungusJournal of fungi (Basel, Switzerland) · 2024Article
- Effects of Lignin-Diverted Reductant with Polyphenol Oxidases on Cellulose Degradation by Wild and Mutant Types of Lytic Polysaccharide Monooxygenase.Current issues in molecular biology · 2024Article
- Lytic polysaccharide monooxygenase synergized with lignin-degrading enzymes for efficient lignin degradation.iScience · 2023Article
- Molecular Mechanism of Substrate Oxidation in Lytic Polysaccharide Monooxygenases: Insight from Theoretical Investigations.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Review
- Recent Advancements and Challenges in Lignin Valorization: Green Routes towards Sustainable Bioproducts.Molecules (Basel, Switzerland) · 2022Review
- Article
- pH Distribution along Growing Fungal Hyphae at Microscale.Journal of fungi (Basel, Switzerland) · 2022Article
- Inhibition of the Peroxygenase Lytic Polysaccharide Monooxygenase by Carboxylic Acids and Amino Acids.Antioxidants (Basel, Switzerland) · 2022Article
- Basidiomycota Fungi and ROS: Genomic Perspective on Key Enzymes Involved in Generation and Mitigation of Reactive Oxygen Species.Frontiers in fungal biology · 2022Review
- Isolation and Characterization of a Novel Laccase for Lignin Degradation, LacZ1.Applied and environmental microbiology · 2021Article
- Oxidative Power: Tools for Assessing LPMO Activity on Cellulose.Biomolecules · 2021Review
- Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide.Bioresources and bioprocessing · 2021Article
- Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600.Biotechnology for biofuels · 2021Article
- Colorimetric LPMO assay with direct implication for cellulolytic activity.Biotechnology for biofuels · 2021Article
- Recent Advances in Screening Methods for the Functional Investigation of Lytic Polysaccharide Monooxygenases.Frontiers in chemistry · 2021Review
- Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe discovery of lignin as activator for the redox enzyme lytic polysaccharide monooxygenases (LPMOs) for the oxidation of cell-wall polysaccharides opens a new scenario for investigation of the interplay between different lignocellulose-degrading enzymes. The lignin-active enzymes in one hand, and the carbohydrate active in the other, are linked through a variety of electrons carrier molecules either derived from lignin or enzymatically transferred. Likewise, in nature, many lignocellulose-degrading organisms are expressing those enzymes simultaneously, and we wanted to test if a major commercial available lignin oxidase enzyme, i.e., laccase could benefit and synergize the activity of the LPMOs by depolymerizing the insoluble lignin.
resultsIn this work, two fungal laccases together with a mediator (ABTS) were used to isolate low-molecular-weight lignin from lignocellulosic biomass. The isolated lignins were used as electron donors for activation of LPMOs. A direct correlation between the low-molecular-weight lignin isolated with laccases and an increased activity of a cellulolytic cocktail containing LPMO was found when pure cellulose was hydrolyzed. We then tried to implement existing commercial cellulases cocktail with laccase enzymes, but under the conditions tested, the co-incubation of laccases with LPMOs showed a substrate competition towards oxygen inhibiting the LPMO. In addition, we found that laccase treatment may cause other modifications to pure cellulose, rendering the material more recalcitrant for enzymatic saccharification.
conclusionsLaccase-mediated system was able to depolymerize lignin from pre-treated and native sugarcane bagasse and wheat straw, and the released phenolic molecules were able to donate electrons to LPMO enzymes boosting the overall enzymatic hydrolysis of cellulose. Likewise, other poly-phenol oxidase, we might have just started showing possible pros or cons in applying several oxidase enzymes for a simultaneous degradation of cellulose and lignin, and we found that the competition towards oxygen and their different consumption rates must be taken into account for any possible co-application.
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