ArticleBiotechnology for biofuels2018
In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Construction of an engineered Escherichia coli strain with enhanced intestinal colonization and anti-inflammatory efficacy in colitis.Cellular & molecular immunology · 2026Article
- Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.Scientific reports · 2026Article
- The engineering toolbox of Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2025Review
- Development of a xylose-inducible and glucose-insensitive expression system for Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2024Article
- The use of thermostable fluorescent proteins for live imaging inFrontiers in microbiology · 2024Article
- Designed active-site library reveals thousands of functional GFP variants.Nature communications · 2023Article
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- A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023Article
- Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli.Microbial cell factories · 2022Article
- New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041.Applied and environmental microbiology · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic CrenarchaeonFrontiers in microbiology · 2021Article
- Live Imaging of a Hyperthermophilic Archaeon Reveals Distinct Roles for Two ESCRT-III Homologs in Ensuring a Robust and Symmetric Division.Current biology : CB · 2020Article
- A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species.Extremophiles : life under extreme conditions · 2020Article
- Multidisciplinary involvement and potential of thermophiles.Folia microbiologica · 2019Review
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundFluorescent reporter proteins (FP) have become an indispensable tool for the optimization of microbial cell factories and in synthetic biology per se. The applicability of the currently available FPs is, however, constrained by species-dependent performance and misfolding at elevated temperatures. To obtain functional reporters for thermophilic, biotechnologically important bacteria such as
resultsFlow cytometry-based benchmarking of a set of GFPs, sfGFPs and species-specific codon-optimized variants revealed that none of the proteins was satisfyingly detectable in
conclusionsThis study illustrates the power of in vivo isolation of thermostable proteins to obtain reporters for highly efficient fluorescence labeling. Successful expression in a variety of thermophilic bacteria proved that the novel FPs are highly suitable for imaging and flow cytometry-based studies. This enables a reliable cell tracking and single-cell-based real-time monitoring of biological processes that are of industrial and biotechnological interest.
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