ReviewReproductive medicine and biology2018
Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9.
Review in Reproductive medicine and biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed.
- Genome Editing for Fertility: Unlocking the Promise of CRISPR/Cas9 in Addressing Male Infertility - A Narrative Review.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis.Development (Cambridge, England) · 2025Article
- Testis-enrichedAmerican journal of translational research · 2025Article
- Individual disruption of 12 testis-enriched genes via the CRISPR/Cas9 system does not affect the fertility of male mice.Journal of reproductive immunology · 2024Article
- Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation.Science China. Life sciences · 2024Article
- Forkhead-associated phosphopeptide binding domain 1 (FHAD1) deficiency impaired murine sperm motility.PeerJ · 2024Article
- Dnali1 is required for sperm motility and male fertility in mice.Basic and clinical andrology · 2023Article
- First-line Evaluation of Sperm Parameters in Mice (Bio-protocol · 2022Article
- The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.Experimental animals · 2022Article
- SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice.Scientific reports · 2021Article
- Analysis of the sperm flagellar axoneme using gene-modified mice.Experimental animals · 2020Review
- CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice†.Biology of reproduction · 2020Article
- Epididymal proteins Binder of SPerm Homologs 1 and 2 (BSPH1/2) are dispensable for male fertility and sperm motility in mice.Scientific reports · 2020Article
- CRISPR/Cas9-Mediated Genome Editing RevealsCells · 2020Article
- The testis-specific expressed gene Spata34 is not required for fertility in mice.Molecular biology reports · 2020Article
- An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility.The Journal of reproduction and development · 2019Article
- Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.American journal of human genetics · 2019Article
- Molecular mechanisms of embryonic implantation in mammals: Lessons from the gene manipulation of mice.Reproductive medicine and biology · 2018Review
- Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9.Reproductive medicine and biology · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Reproductive research is quintessential in understanding not only the cause of infertility, but also for creating family planning tools. The knockout (KO) system approach is conducive to discovering genes that are essential for fertility in mice. However, in vivo research has been limited due to its high cost and length of time needed to establish KO mice. Methods: The mechanisms behind the CRISPR/Cas9 system and its application in investigating male fertility in mice are described by using original and review articles. Results: The CRISPR/CAS9 SYSTEM has enabled researchers to rapidly, efficiently, and inexpensively produce genetically modified mice to study male fertility. Several genes have been highlighted that were found to be indispensable for male fertility by using the CRISPR/Cas9 system, as well as more complicated gene manipulation techniques, such as point mutations, tag insertions, and double knockouts, which have become easier with this new technology. Conclusion: In order to increase efficiency and usage, new methods of CRISPR/Cas9 integration are being developed, such as electroporation and applying the system to embryonic stem cells. The hidden mysteries of male fertility will be unraveled with the help of this new technology.
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Registered trials
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