Evidence map›Paper›PMID 29370578›Full record

ArticleJournal of occupational and environmental hygiene2018

Microbial hazards during harvesting and processing at an outdoor United States cannabis farm.

Brett J Green, James R Couch, Angela R Lemons, Nancy C Burton, Kerton R Victory, Ajay P Nayak, Donald H Beezhold

Open access · greenAbstract read
In one paragraph

Article in Journal of occupational and environmental hygiene, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
8.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

  1. Allergic Anaphylactic Risk in Farming Activities: A Systematic Review.International journal of environmental research and public health · 2020
    Pooled it
  2. Article
  3. Review
  4. Article
  5. Ultraviolet radiation exposure in cannabis-growing facilities.Journal of occupational and environmental hygiene · 2023
    Article
  6. Article
  7. The Emerging Spectrum of Respiratory Diseases in the U.S. Cannabis Industry.Seminars in respiratory and critical care medicine · 2023
    Article
  8. Cannabis: An Emerging Occupational Allergen?Annals of work exposures and health · 2020
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Interpol review of controlled substances 2016-2019.Forensic science international. Synergy · 2020
    Review
  15. Article
  16. Emerging Insights into the Occupational Mycobiome.Current allergy and asthma reports · 2018
    Review
  17. Occupational Allergies to Cannabis.The journal of allergy and clinical immunology. In practice
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Brett J Greena Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Morgantown , West Virginia.
James R Couchb Hazard Evaluations and Technical Assistance Branch, Division of Surveillance, Hazard Evaluations and Field Studies, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Cincinnati , Ohio.
Angela R Lemonsa Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Morgantown , West Virginia.
Nancy C Burtonb Hazard Evaluations and Technical Assistance Branch, Division of Surveillance, Hazard Evaluations and Field Studies, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Cincinnati , Ohio.
Kerton R Victoryc Office of the Director, Emergency Preparedness and Response Office, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Atlanta , Georgia.
Ajay P Nayaka Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Morgantown , West Virginia.
Donald H Beezholdd Office of the Director, Health Effects Laboratory Division, National Institute for Occupational Safety and Health , Centers for Disease Control and Prevention , Morgantown , West Virginia.
Centers for Disease Control and Prevention · USNational Institute for Occupational Safety and Health · USOffice of the Director · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Cannabis cultivation is an emerging industry within the United States. Organic dust derived in part from naturally occurring microorganisms is known to cause byssinosis in the hemp industry. In this pilot study, bacteria and fungi encountered by workers at an outdoor cannabis farm that utilized organic practices were elucidated by 16 S ribosomal RNA (rRNA) and Internal Transcribed Spacer (ITS) region sequencing, respectively. Area (n = 14) and personal air samples (n = 12) were collected during harvesting and processing activities. 16 S rRNA and ITS regions of extracted bacterial and fungal genomic DNA were amplified and sequenced using Sanger sequencing. Bacterial sequencing resolved 1,077 sequences that were clustered into 639 operational taxonomic units (OTUs) and predominantly placed in the phylum, Actinobacteria (46%). Personal air samples revealed higher bacterial and Actinobacteria diversity compared to outdoor area samples collected within the facility (p < 0.05). A high degree of dissimilarity between bacteria was identified within and between samples. Fungal sequences (n = 985) were identified and predominantly clustered in the phylum Ascomycota (53%). Of the 216 fungal OTUs elucidated, the cannabis plant pathogenic species, Botrytis cinerea, was the most prevalent and accounted for 34% of all fungal sequences. The relative abundance of B. cinerea was highest in personal air samples (59%) compared to area samples collected in the drying room (19%), greenhouse (18%), and outdoor environment (6%). There was 49% sample similarity between fungi identified within personal air samples, but higher dissimilarity coefficients were observed within and between greenhouse, drying room, and outdoor area air samples. The results of this pilot study suggest that the cannabis farm workers are potentially exposed to Actinobacteria as well as the cannabis plant pathogen, B. cinerea during harvesting, bud-stripping, and hand-trimming processes.

Indexed as

CannabisAir MicrobiologyBacteriaBotrytisDNA, BacterialDNA, FungalDNA, Ribosomal SpacerFarmersFungiHumansNational Institute for Occupational Safety and Health, U.S.Occupational ExposurePilot ProjectsRNA, Ribosomal, 16SUnited StatesWashingtonDNA, BacterialDNA, FungalDNA, Ribosomal SpacerRNA, Ribosomal, 16SBacteriacannabisexposure assessmentfungusgene sequencingoccupational hazards

Identifiers

PMID29370578
PMCPMC6314012
OpenAlexW2784462991

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.