Evidence map›Paper›PMID 29370558›Full record

ArticleCancer biology & therapy2018

Pim-3 enhances melanoma cell migration and invasion by promoting STAT3 phosphorylation.

Jing Liu, Xinyu Qu, Liwei Shao, Yuan Hu, Xin Yu, Peixiang Lan, Qie Guo, Qiuju Han, Jian Zhang, Cai Zhang

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biology & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Jing Liua Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Xinyu Qua Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Liwei Shaoa Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Yuan Hua Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Xin Yua Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Peixiang Lana Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Qie Guoa Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Qiuju Hana Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Jian Zhanga Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Cai Zhanga Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University , Jinan , Shandong , China.
Institute of Immunology · HRShandong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Melanoma is the deadliest form of commonly encountered skin cancer, and has fast propagating and highly invasive characteristics. Pim-3, a highly expressed oncogene in melanoma, is a highly conserved serine/threonine kinase with various biological activities, such as proliferation-accelerating and anti-apoptosis effects on cancer progression. However, whether Pim-3 regulates melanoma metastasis has not been determined. Here, we constructed a Pim-3-silencing short hairpin RNA (sh-Pim-3), a TLR7-stimulating ssRNA and a dual-function vector containing a sh-Pim-3 and a ssRNA, and transfected them into the B16F10 melanoma cell line to investigate the effects of Pim-3 on migration and invasion in melanoma. We found that sh-Pim-3 inhibited B16F10 cell migration and invasion in vitro. In a tumor-bearing mouse model, sh-Pim-3 significantly downregulated pulmonary metastasis of B16F10 melanoma cell in vivo. Mechanistically, sh-Pim-3 inhibited metastasis by regulating the expression of genes related to epithelial-mesenchymal transition (EMT). Further study revealed that by promoting the phosphorylation of STAT3 (signal transducer and activator of transcription 3), Pim-3 induced the expression of Slug, Snail, and ZEB1, which enhanced EMT-related changes and induced melanoma migration and invasion. Our study suggests that Pim-3 is a potential effective target for melanoma therapy.

Indexed as

AnimalsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansLungLung NeoplasmsMelanoma, ExperimentalMiceMice, Inbred C57BLNeoplasm InvasivenessPhosphorylationProtein BindingProtein Serine-Threonine KinasesProto-Oncogene ProteinsPim3 protein, mouseProtein Serine-Threonine KinasesProto-Oncogene ProteinsRNA, Small InterferingStat3 protein, mouseSTAT3 Transcription FactorEMTMelanomaMetastasisPim-3p-STAT3

Identifiers

PMID29370558
PMCPMC5790343
OpenAlexW2792536497

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.