ArticleZebrafish2018
Changes to Extender, Cryoprotective Medium, and In Vitro Fertilization Improve Zebrafish Sperm Cryopreservation.
Article in Zebrafish, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- CompletebioRxiv : the preprint server for biology · 2025Article
- Integrated Studies on Male Reproductive Toxicity of Decabromodiphenyl Ethane in Zebrafish SpermatozoaEnvironmental health perspectives · 2024Article
- Cholesterol-loaded cyclodextrin improves motility and survival of cryopreserved zebrafish (Danio rerio) sperm.Cryobiology · 2024Article
- Modeling of cryopreservation pathway operation at an aquatic biomedical stock center for zebrafish.Cryobiology · 2023Article
- Optimization of a non-activating medium for short-term chilled storage of barramundi (Lates calcarifer) testicular spermatozoa.Fish physiology and biochemistry · 2023Article
- Initial assessment of the toxicologic effects of leachates from 3-dimensional (3-D) printed objects on sperm quality in two model fish species.Aquatic toxicology (Amsterdam, Netherlands) · 2023Article
- A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone.Zebrafish · 2022Review
- Cryopreservation ofAnimals : an open access journal from MDPI · 2022Article
- Differential reproductive plasticity under thermal variability in a freshwater fish (Proceedings. Biological sciences · 2022Article
- Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?Cryobiology · 2022Article
- Effect of egg yolk of free-range chicken and methanol as a cryoprotective agent for the sperm preservation of cyprinid fish,Heliyon · 2021Article
- A 3D Printed Vitrification Device for Storage in Cryopreservation Vials.Applied sciences (Basel, Switzerland) · 2021Article
- Optimization of Sperm Management and Fertilization in Zebrafish (Animals : an open access journal from MDPI · 2021Article
- Postembryonic screen for mutations affecting spine development in zebrafish.Developmental biology · 2021Article
- Cryopreservation of Pooled Sperm Samples.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cryopreservation and Transplantation of Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cryopreservation and Laser Nanowarming of Zebrafish Embryos Followed by Hatching and Spawning.Advanced biosystems · 2020Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Sperm cryopreservation is a highly efficient method for preserving genetic resources. It extends the reproductive period of males and significantly reduces costs normally associated with maintenance of live animal colonies. However, previous zebrafish (Danio rerio) cryopreservation methods have produced variable outcomes and low post-thaw fertilization rates. To improve post-thaw fertilization rates after cryopreservation, we developed a new extender and cryoprotective medium (CPM), introduced quality assessment (QA), determined the optimal cooling rate, and improved the post-thaw in vitro fertilization process. We found that the hypertonic extender E400 preserved motility of sperm held on ice for at least 6 h. We implemented QA by measuring sperm cell densities with a NanoDrop spectrophotometer and sperm motility with computer-assisted sperm analysis (CASA). We developed a CPM, RMMB, which contains raffinose, skim milk, methanol, and bicine buffer. Post-thaw motility indicated that the optimal cooling rate in two types of cryogenic vials was between 10 and 15°C/min. Test thaws from this method produced average motility of 20% ± 13% and an average post-thaw fertilization rate of 68% ± 16%.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.