ArticleApplied microbiology and biotechnology2018
Inducer-independent production of pectinases in Aspergillus niger by overexpression of the D-galacturonic acid-responsive transcription factor gaaR.
Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 43 citations in OpenAlex.
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- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Evaluation ofJournal of fungi (Basel, Switzerland) · 2022Article
- Discovery of novel secretome CAZymes fromFrontiers in bioengineering and biotechnology · 2022Article
- Integrated engineering of enzymes and microorganisms for improving the efficiency of industrial lignocellulose deconstruction.Engineering microbiology · 2021Review
- CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars.Biotechnology for biofuels · 2021Article
- Loss of function of the carbon catabolite repressor CreA leads to low but inducer-independent expression from the feruloyl esterase B promoter in Aspergillus niger.Biotechnology letters · 2021Article
- Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain.Biotechnology letters · 2021Article
- Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization inFrontiers in fungal biology · 2021Article
- Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus.Biotechnology for biofuels · 2020Article
- Comparative Characterization ofFrontiers in bioengineering and biotechnology · 2020Article
- W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.MicrobiologyOpen · 2019Article
- Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.Microbial cell factories · 2019Review
- Modulating Transcriptional Regulation of Plant Biomass Degrading Enzyme Networks for Rational Design of Industrial Fungal Strains.Frontiers in bioengineering and biotechnology · 2018Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The transcription factor GaaR is needed for the expression of genes required for pectin degradation and transport and catabolism of the main degradation product, D-galacturonic acid (GA) in Aspergillus niger. In this study, we used the strong constitutive gpdA promoter of Aspergillus nidulans to overexpress gaaR in A. niger. Overexpression of gaaR resulted in an increased transcription of the genes encoding pectinases, (putative) GA transporters, and catabolic pathway enzymes even under non-inducing conditions, i.e., in the absence of GA. Exoproteome analysis of a strain overexpressing gaaR showed that this strain secretes highly elevated levels of pectinases when grown in fructose. The genes encoding exo-polygalacturonases were found to be subjected to CreA-mediated carbon catabolite repression, even in the presence of fructose. Deletion of creA in the strain overexpressing gaaR resulted in a further increase in pectinase production in fructose. We showed that GaaR localizes mainly in the nucleus regardless of the presence of an inducer, and that overexpression of gaaR leads to an increased concentration of GaaR in the nucleus.
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