Evidence map›Paper›PMID 29368143›Full record

ArticleIn vitro cellular & developmental biology. Animal2018

Expression of three reporter genes in four cell lines developed from Papilio demoleus Linnaeus (Lepidoptera: Papilionidae).

Wei-Feng Ding, Xin Zhang, Xian Li, Zhi-Gang Liu, Shi-Cong Xie, Ying Feng

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Wei-Feng DingResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China.
Xin ZhangResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China.
Xian LiResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China.
Zhi-Gang LiuResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China.
Shi-Cong XieResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China.
Ying FengResearch Institute of Resource Insects, Chinese Academy of Forestry, the Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, Yunnan, 650233, China. rirify@139.com.
Chinese Academy of Forestry · CNResearch Institute of Resource Insects · CN

Funding

the Fundamental Research Funds for the Central Non-profit Research Institution of CAF CAFYBB2016ZD005
6 · The paper itself

Abstract

This paper used recombinant baculoviruses that carried three reporter genes, green fluorescent protein (GFP), β-galactosidase, and secreted alkaline phosphatase (SEAP), to infect four new cell lines from Papilio demoleus Linnaeus larvae (named RIRI-PaDe-1, RIRI-PaDe-2, RIRI-PaDe-3, and RIRI-PaDe-4). The expression levels of the three recombinant proteins were detected at 24, 48, 72, 96, 120, and 144 h after infection and compared with Sf9 and High Five cells to evaluate the characteristics of these four cell lines as host cells. The inoculation densities of the tested cell lines were 2 × 10

Indexed as

Alkaline PhosphataseAnimalsBaculoviridaebeta-GalactosidaseCell Culture TechniquesCells, CulturedGenes, ReporterGreen Fluorescent ProteinsLepidopteraRecombinant ProteinsAlkaline Phosphatasebeta-GalactosidaseGreen Fluorescent ProteinsRecombinant ProteinsBaculovirus expression systemInsect cell linePapilio demoleus LinnaeusRecombinant protein

Identifiers

PMID29368143
OpenAlexW2785197532

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.