ArticleMetabolomics : Official journal of the Metabolomic Society2018
A comparison of sample preparation methods for extracting volatile organic compounds (VOCs) from equine faeces using HS-SPME.
Article in Metabolomics : Official journal of the Metabolomic Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04995809 (Investigating the Effect of Pelvic Radiotherapy on the Intestinal Microbiome and Metabolome to Improve the Detection and Management of Gastrointestinal), which is not on this map. Cited by 15 papers.
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Investigating the Effect of Pelvic Radiotherapy on the Intestinal Microbiome and Metabolome to Improve the Detection and Management of Gastrointestinal (GI) Toxicity.
Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
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- Distinct Profiles of Fecal Volatile Organic Compounds Discriminate Ulcerative Colitis Patients With an Ileoanal Pouch From Those With an Intact Colon.Molecular nutrition & food research · 2025Article
- Gas Chromatography-Mass Spectrometry-Based Analyses of Fecal Short-Chain Fatty Acids (SCFAs): A Summary Review and Own Experience.Biomedicines · 2024Review
- Recent advances in measuring the effects of diet on gastrointestinal physiology: Sniffing luminal gases and fecal volatile organic compounds.JGH open : an open access journal of gastroenterology and hepatology · 2024Review
- Optimization and validation of an HS-SPME/GC-MS method for determining volatile organic compounds in dry-cured ham.Frontiers in nutrition · 2024Article
- GC-MS Techniques Investigating Potential Biomarkers of Dying in the Last Weeks with Lung Cancer.International journal of molecular sciences · 2023Article
- Review
- Links Between Feeding Preferences and Electroantennogram Response Profiles in Dung Beetles: The Importance of Dung Odor Bouquets.Journal of chemical ecology · 2022Article
- A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome ofAnimals : an open access journal from MDPI · 2021Article
- The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling.International journal of molecular sciences · 2021Review
- Article
- Differences in Compositions of Gut Bacterial Populations and Bacteriophages in 5-11 Year-Olds Born Preterm Compared to Full Term.Frontiers in cellular and infection microbiology · 2020Article
- Acute changes in the colonic microbiota are associated with large intestinal forms of surgical colic.BMC veterinary research · 2019Article
- Global Plasma Profiling for Colorectal Cancer-Associated Volatile Organic Compounds: a Proof-of-Principle Study.Journal of chromatographic science · 2019Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDisturbance to the hindgut microbiota can be detrimental to equine health. Metabolomics provides a robust approach to studying the functional aspect of hindgut microorganisms. Sample preparation is an important step towards achieving optimal results in the later stages of analysis. The preparation of samples is unique depending on the technique employed and the sample matrix to be analysed. Gas chromatography mass spectrometry (GCMS) is one of the most widely used platforms for the study of metabolomics and until now an optimised method has not been developed for equine faeces.
objectivesTo compare a sample preparation method for extracting volatile organic compounds (VOCs) from equine faeces.
methodsVolatile organic compounds were determined by headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GCMS). Factors investigated were the mass of equine faeces, type of SPME fibre coating, vial volume and storage conditions.
resultsThe resultant method was unique to those developed for other species. Aliquots of 1000 or 2000 mg in 10 ml or 20 ml SPME headspace were optimal. From those tested, the extraction of VOCs should ideally be performed using a divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS) SPME fibre. Storage of faeces for up to 12 months at - 80 °C shared a greater percentage of VOCs with a fresh sample than the equivalent stored at - 20 °C.
conclusionsAn optimised method for extracting VOCs from equine faeces using HS-SPME-GCMS has been developed and will act as a standard to enable comparisons between studies. This work has also highlighted storage conditions as an important factor to consider in experimental design for faecal metabolomics studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.