ArticleScientific reports2018
An optimised protocol for platelet-rich plasma preparation to improve its angiogenic and regenerative properties.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04320498 (Effects of Platelet-rich Plasma in the Treatment of Chronic Anal Fissure), which is not on this map. Cited by 66 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effects of Platelet-rich Plasma in the Treatment of Chronic Anal Fissure
Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it, 134 citations in OpenAlex.
- Intravesical Platelet-Rich Plasma Injection for Refractory Interstitial Cystitis/Painful Bladder Syndrome: A Systematic Review and Meta-analysis.International urogynecology journal · 2026Pooled it
- Photothermal-Activated Platelet-Rich Plasma Versus Conventional Platelet-Rich Plasma for Melasma: A Split-Face, Double-Blind Randomized Controlled Trial.Photodermatology, photoimmunology & photomedicine · 2026Trial
- Repeated intravesical injections of platelet-rich plasma improve symptoms and alter urinary functional proteins in patients with refractory interstitial cystitis.Scientific reports · 2020Trial
- Protocol for ex vivo physicochemical assessment of photothermally preconditioned platelet-rich plasma.MethodsX · 2026Article
- Current understanding and new promises of umbilical cord blood in emerging clinical applications.Science China. Life sciences · 2026Review
- Effect of intraoperative platelet-rich plasma on inferior alveolar nerve neurosensory outcomes after high-risk mandibular third molar extraction.Medicina oral, patologia oral y cirugia bucal · 2026Article
- Photothermal preconditioning of platelet-rich plasma: An emerging regenerative strategy.Regenerative therapy · 2026Article
- Platelet-rich Plasma: ACurrent drug research reviews · 2026Review
- Platelet-rich plasma for the treatment of a pressure wound: a case report and review of the literature.Frontiers in bioengineering and biotechnology · 2026Article
- Microvascular Health as a Key Determinant of Organismal Aging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- A Design of Experiments Approach to Identify Critical Processing Parameters for Manufacture of an Autologous Platelet Gel for Diabetic Foot Ulcer.Pharmaceutics · 2025Article
- A Prospective Study Assessing Functional Outcomes of Arthroscopic Subacromial Decompression with Platelet-Rich Plasma Augmentation in Shoulder Impingement Syndrome.Malaysian orthopaedic journal · 2025Article
- Platelet-rich plasma as a potential antimicrobial agent against multidrug-resistant bacteria in diabetic foot infections.Scientific reports · 2025Article
- Platelet like cells differentiated from human adipose derived mesenchymal stem cells promote healing of tendinopathy in rats.Scientific reports · 2025Article
- Platelet-rich plasma and plasma rich in growth factors in extra-oral wound care.Periodontology 2000 · 2025Review
- A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing.Frontiers in bioengineering and biotechnology · 2025Article
- Platelet-Rich Plasma in Diabetic Foot Ulcer Healing: Contemplating the Facts.International journal of molecular sciences · 2024Review
- Hypoxia Preconditioned Serum Hydrogel (HPS-H) Accelerates Dermal Regeneration in a Porcine Wound Model.Gels (Basel, Switzerland) · 2024Article
- The Biological Effect of Enriching the Plasma Content in Platelet-Rich Plasma: An In Vitro Study.Biomolecules · 2024Article
- Survival Mechanisms and Retention Strategies in Large-Volume Fat Grafting: A Comprehensive Review and Future Perspectives.Aesthetic plastic surgery · 2024Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although platelet-rich plasma (PRP) is used as a source of growth factors in regenerative medicine, its effectiveness remains controversial, partially due to the absence of PRP preparation protocols based on the regenerative role of platelets. Here, we aimed to optimise the protocol by analysing PRP angiogenic and regenerative properties. Three optimising strategies were evaluated: dilution, 4 °C pre-incubation, and plasma cryoprecipitate supplementation. Following coagulation, PRP releasates (PRPr) were used to induce angiogenesis in vitro (HMEC-1 proliferation, migration, and tubule formation) and in vivo (chorioallantoic membrane), as well as regeneration of excisional wounds on mouse skin. Washed platelet releasates induced greater angiogenesis than PRPr due to the anti-angiogenic effect of plasma, which was decreased by diluting PRPr with saline. Angiogenesis was also improved by both PRP pre-incubation at 4 °C and cryoprecipitate supplementation. A combination of optimising variables exerted an additive effect, thereby increasing the angiogenic activity of PRPr from healthy donors and diabetic patients. Optimised PRPr induced faster and more efficient mouse skin wound repair compared to that induced by non-optimised PRPr. Acetylsalicylic acid inhibited angiogenesis and tissue regeneration mediated by PRPr; this inhibition was reversed following optimisation. Our findings indicate that PRP pre-incubation at 4 °C, PRPr dilution, and cryoprecipitate supplementation improve the angiogenic and regenerative properties of PRP compared to the obtained by current methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.