ArticleMolecules (Basel, Switzerland)2018
Informing Efforts to Develop Nitroreductase for Amine Production.
Article in Molecules (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Nitroreductase-triggered indazole formation.Nature communications · 2026Article
- Biocatalytic reduction of nitro-sulfonamides: enzyme engineering, process integration, and sustainable pathways toFrontiers in bioengineering and biotechnology · 2026Review
- Real-Time Detection of Reduced Nitroreductase with a Reversible Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Novel TdsD nitroreductase: characterization of kinetics and substrate specificity.Biotechnology letters · 2025Article
- Tailored photoenzymatic systems for selective reduction of aliphatic and aromatic nitro compounds fueled by light.Nature communications · 2023Article
- Oxygen-insensitive nitroreductase E. coli NfsA, but not NfsB, is inhibited by fumarate.Proteins · 2023Article
- Sequence Conservation Does Not Always Signify a Functional Imperative as Observed in the Nitroreductase Superfamily.Biochemistry · 2022Article
- The minimal structure for iodotyrosine deiodinase function is defined by an outlier protein from the thermophilic bacterium Thermotoga neapolitana.The Journal of biological chemistry · 2021Article
- Single- and Two-Electron Reduction of Nitroaromatic Compounds by Flavoenzymes: Mechanisms and Implications for Cytotoxicity.International journal of molecular sciences · 2021Review
- A Review of Chemical Tools for Studying Small Molecule Persulfides: Detection and Delivery.ACS chemical biology · 2021Review
- Targeted Delivery of Persulfides to the Gut: Effects on the Microbiome.Angewandte Chemie (International ed. in English) · 2021Article
- Degradation of High Energy Materials Using Biological Reduction: A Rational Way to Reach Bioremediation.International journal of molecular sciences · 2019Article
- Synthesis and Biological Evaluation of Lipophilic Nucleoside Analogues as Inhibitors of Aminoacyl-tRNA Synthetases.Antibiotics (Basel, Switzerland) · 2019Article
- Antibacterial Activity and Mode of Action of a Sulfonamide-Based Class of Oxaborole Leucyl-tRNA-Synthetase Inhibitors.ACS infectious diseases · 2019Article
- Special Issue: Flavoenzymes.Molecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitroreductases (NRs) hold promise for converting nitroaromatics to aromatic amines. Nitroaromatic reduction rate increases with Hammett substituent constant for NRs from two different subgroups, confirming substrate identity as a key determinant of reactivity. Amine yields were low, but compounds yielding amines tend to have a large π system and electron withdrawing substituents. Therefore, we also assessed the prospects of varying the enzyme. Several different subgroups of NRs include members able to produce aromatic amines. Comparison of four NR subgroups shows that they provide contrasting substrate binding cavities with distinct constraints on substrate position relative to the flavin. The unique architecture of the NR dimer produces an enormous contact area which we propose provides the stabilization needed to offset the costs of insertion of the active sites between the monomers. Thus, we propose that the functional diversity included in the NR superfamily stems from the chemical versatility of the flavin cofactor in conjunction with a structure that permits tremendous active site variability. These complementary properties make NRs exceptionally promising enzymes for development for biocatalysis in prodrug activation and conversion of nitroaromatics to valuable aromatic amines. We provide a framework for identifying NRs and substrates with the greatest potential to advance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.