ArticleJournal of visualized experiments : JoVE2018
Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 14 citations in OpenAlex.
- Nanoparticle-based approaches for wound healing: a comprehensive review of nanomaterials enhancing tissue regeneration.Discover nano · 2025Review
- Distinct roles of amniotic membrane epithelial (hAEC) and mesenchymal stromal cells (hAMSC) in amniotic membrane-driven wound healing.Scientific reports · 2025Article
- Refrigerated amniotic membrane maintains its therapeutic qualities for 48 hours.Frontiers in bioengineering and biotechnology · 2024Article
- Oleanolic Acid Complexation with Cyclodextrins Improves Its Cell Bio-Availability and Biological Activities for Cell Migration.International journal of molecular sciences · 2023Article
- In Vitro Approaches to Determine the Potential Carcinogenic Risk of Environmental Pollutants.International journal of molecular sciences · 2023Review
- Oleanolic acid rescues critical features of umbilical vein endothelial cells permanently affected by hyperglycemia.Frontiers in endocrinology · 2023Article
- Oleanolic acid stimulation of cell migration involves a biphasic signaling mechanism.Scientific reports · 2022Article
- Disruption of Toxoplasma gondii-Induced Host Cell DNA Replication Is Dependent on Contact Inhibition and Host Cell Type.mSphere · 2022Article
- Perinatal derivatives: How to best characterize their multimodal functionsFrontiers in bioengineering and biotechnology · 2022Review
- Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.PloS one · 2021Review
- The immunologic changes during different phases of intestinal anastomotic healing.Journal of clinical laboratory analysis · 2020Review
- Nicotine Impairs the Response of Lung Epithelial Cells to IL-22.Mediators of inflammation · 2020Article
- In vitro cell migration quantification method for scratch assays.Journal of the Royal Society, Interface · 2019Article
- Antiproliferative and apoptotic effects of conditioned medium released from human amniotic epithelial stem cells on breast and cervical cancer cells.International journal of immunopathology and pharmacologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration is a mandatory aspect for wound healing. Creating artificial wounds on research animal models often results in costly and complicated experimental procedures, while potentially lacking in precision. In vitro culture of epithelial cell lines provides a suitable platform for researching the cell migratory behavior in wound healing and the impact of treatments on these cells. The physiology of epithelial cells is often studied in non-confluent conditions; however, this approach may not resemble natural wound healing conditions. Disrupting the epithelium integrity by mechanical means generates a realistic model, but may impede the application of molecular techniques. Consequently, microscopy based techniques are optimal for studying epithelial cell migration in vitro. Here we detail two specific methods, the artificial wound scratch assay and the artificial migration front assay, that can obtain quantitative and qualitative data, respectively, on the migratory performance of epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.