ArticleJournal of visualized experiments : JoVE2018
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolism.Translational neurodegeneration · 2026Article
- Functional FTSH4 complexes in Arabidopsis mitochondria: a megacomplex with SLP1 and SLP1-free smaller complexes.Plant & cell physiology · 2026Article
- Dysregulated 3'-end processing of 18S pre-rRNA decreases mtPNPase efficiency in plant mitochondria.Nucleic acids research · 2026Article
- Onion-Mitochondria Inhibit Lipopolysaccharide-Induced Acute Lung Injury by Shaping Lung Macrophage Mitochondrial Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Coordinated regulation of the mitochondrial retrograde response by circadian clock regulators and ANAC017.Plant communications · 2023Article
- A novelFrontiers in plant science · 2023Article
- FTSH4 and OMA1 mitochondrial proteases reduce moderate heat stress-induced protein aggregation.Plant physiology · 2021Article
- Mitochondrial RNase H1 activity regulates R-loop homeostasis to maintain genome integrity and enable early embryogenesis in Arabidopsis.PLoS biology · 2021Article
- Conserved and Opposite Transcriptome Patterns during Germination inInternational journal of molecular sciences · 2020Article
- Joint inhibition of mitochondrial complex IV and alternative oxidase by genetic or chemical means represses chloroplast transcription inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Article
- Functional Characterisation of Alternative Oxidase Protein Isoproteins in Arabidopsis thaliana.Physiologia plantarumArticle
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria are essential organelles involved in numerous metabolic pathways in plants, most notably the production of adenosine triphosphate (ATP) from the oxidation of reduced compounds such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FADH2). The complete annotation of the Arabidopsis thaliana genome has established it as the most widely used plant model system, and thus the need to purify mitochondria from a variety of organs (leaf, root, or flower) is necessary to fully utilize the tools that are now available for Arabidopsis to study mitochondrial biology. Mitochondria are isolated by homogenization of the tissue using a variety of approaches, followed by a series of differential centrifugation steps producing a crude mitochondrial pellet that is further purified using continuous colloidal density gradient centrifugation. The colloidal density material is subsequently removed by multiple centrifugation steps. Starting from 100 g of fresh leaf tissue, 2 - 3 mg of mitochondria can be routinely obtained. Respiratory experiments on these mitochondria display typical rates of 100 - 250 nmol O2 min
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