ArticleJournal of visualized experiments : JoVE2018
Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed.
- Quantitative ethology of schistosome miracidia characterizes a conserved snail peptide that inhibits host recognition.PLoS pathogens · 2025Article
- Evaluation of Suitable Reference Gene During the Development of Paired or Unpaired FemalePathogens (Basel, Switzerland) · 2025Article
- A close unicellular animal relative and predator of schistosomes exhibits chemokinesis in response to proteins and peptides from its prey.PLoS pathogens · 2025Article
- Chemokinesis by a microbial predator.bioRxiv : the preprint server for biology · 2025Article
- A graphical user interface for wrmXpress 2.0 streamlines helminth phenotypic screening.International journal for parasitology. Drugs and drug resistance · 2025Article
- Single-cell transcriptomics of the human parasite Schistosoma mansoni first intra-molluscan stage reveals tentative tegumental and stem-cell regulators.Scientific reports · 2024Article
- Parasite transmission in size-structured populations.Ecology · 2024Article
- The Truman Show for Human Helminthic Parasites: A Review of Recent Advances in In Vitro Cultivation Platforms.Microorganisms · 2023Review
- Phenotypic Profiling of Macrocyclic Lactones on ParasiticAntimicrobial agents and chemotherapy · 2023Article
- Proteomic Analysis ofFrontiers in immunology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Human blood flukes, Schistosoma spp., have a complex life cycle that involves asexual and sexual developmental phases within a snail intermediate and mammalian final host, respectively. The ability to isolate and sustain the different life cycle stages under in vitro culture conditions has greatly facilitated investigations of the cellular, biochemical and molecular mechanisms regulating parasite growth, development and host interactions. Transmission of schistosomiasis requires asexual reproduction and development of multiple larval stages within the snail host; from the infective miracidium, through primary and secondary sporocysts, to the final cercarial stage that is infective to humans. In this paper we present a step-by-step protocol for mass hatching and isolation of Schistosoma mansoni miracidia from eggs obtained from livers of infected mice, and their subsequent introduction into in vitro culture. It is anticipated that the detailed protocol will encourage new researchers to engage in and broaden this important field of schistosome research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.